DNA sequencing by capillary electrophoresis (review)

被引：55
作者：
Dolník, V [1 ]
机构：
[1] Mol Dynam, Sunnyvale, CA 94086 USA
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1999年 / 41卷 / 2-3期
关键词：
DNA sequencing; capillary electrophoresis; sieving matrix; entangled polymer; wall coating;
D O I：
10.1016/S0165-022X(99)00041-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA sequencing by capillary electrophoresis has been reviewed with an emphasis on progress during the last four years. The effects of sample purification, composition of sieving matrices, electric field strength, temperature, wall coating and DNA labeling on the DNA sequencing performance are discussed. Multicapillary array instrumentation is compared with one-capillary systems. Integrated systems that perform the whole DNA sequencing operation online starting from the DNA amplification through base calling and data processing are discussed. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：103 / 119
页数：17
相关论文
共 112 条
[1]   A capillary array gel electrophoresis system using multiple laser focusing for DNA sequencing [J].
Anazawa, T ;
Takahashi, S ;
Kambara, H .
ANALYTICAL CHEMISTRY, 1996, 68 (15) :2699-2704
[2]   POLYMERIC SEPARATION MEDIA FOR ELECTROPHORESIS - CROSS-LINKED SYSTEMS OR ENTANGLED SOLUTIONS [J].
BAE, YC ;
SOANE, D .
JOURNAL OF CHROMATOGRAPHY A, 1993, 652 (01) :17-22
[3]  
Bashkin J, 1996, APPL THEOR ELECTROPH, V6, P23
[4]  
BASHKIN J, 1999, HPCE 99
[5]  
Bashkin JS, 1996, J CAPILLARY ELECTROP, V3, P61
[6]   SEPARATION OF FRAGMENTS UP TO 570 BASES IN LENGTH BY USE OF 6-PERCENT T-NON-CROSS-LINKED POLYACRYLAMIDE FOR DNA-SEQUENCING IN CAPILLARY ELECTROPHORESIS [J].
BEST, N ;
ARRIAGA, E ;
CHEN, DY ;
DOVICHI, NJ .
ANALYTICAL CHEMISTRY, 1994, 66 (22) :4063-4067
[7]   DNA sequencing after the Human Genome Project [J].
Cantor, CR ;
Tang, K ;
Graber, JH ;
Maloney, M ;
Fu, DJ ;
Broude, NE ;
Siddiqi, F ;
Koester, H ;
Smith, CL .
NUCLEOSIDES & NUCLEOTIDES, 1997, 16 (5-6) :591-598
[8]   Rapid DNA sequencing of more than 1000 bases per run by capillary electrophoresis using replaceable linear polyacrylamide solutions [J].
Carrilho, E ;
RuizMartinez, MC ;
Berka, J ;
Smirnov, I ;
Goetzinger, W ;
Miller, AW ;
Brady, D ;
Karger, BL .
ANALYTICAL CHEMISTRY, 1996, 68 (19) :3305-3313
[9]   DNA-SEQUENCING BY CAPILLARY ELECTROPHORESIS - USE OF A 2-LASER 2-WINDOW INTENSIFIED DIODE-ARRAY DETECTION SYSTEM [J].
CARSON, S ;
COHEN, AS ;
BELENKII, A ;
RUIZMARTINEZ, MC ;
BERKA, J ;
KARGER, BL .
ANALYTICAL CHEMISTRY, 1993, 65 (22) :3219-3226
[10]   POLY(ETHYLENEOXIDE) FOR HIGH-RESOLUTION AND HIGH-SPEED SEPARATION OF DNA BY CAPILLARY ELECTROPHORESIS [J].
CHANG, HT ;
YEUNG, ES .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1995, 669 (01) :113-123
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