Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

被引：264

作者：

Moller, Peter ^{[1
]}

Azqueta, Amaya ^{[2
,3
]}

Boutet-Robinet, Elisa ^{[4
]}

Koppen, Gudrun ^{[5
]}

Bonassi, Stefano ^{[6
,7
]}

Milic, Mirta ^{[8
]}

Gajski, Goran ^{[8
]}

Costa, Solange ^{[9
,10
]}

Teixeira, Joao Paulo ^{[9
,10
]}

Costa Pereira, Cristiana ^{[9
,10
]}

Dusinska, Maria ^{[11
]}

Godschalk, Roger ^{[12
]}

Brunborg, Gunnar ^{[13
]}

Gutzkow, Kristine B. ^{[13
]}

Giovannelli, Lisa ^{[14
]}

Cooke, Marcus S. ^{[15
]}

Richling, Elke ^{[16
]}

Laffon, Blanca ^{[17
,18
]}

Valdiglesias, Vanessa ^{[17
,18
]}

Basaran, Nursen ^{[19
]}

Del Bo', Cristian ^{[20
]}

Zegura, Bojana ^{[21
]}

Novak, Matjaz ^{[21
]}

Stopper, Helga ^{[22
]}

Vodicka, Pavel ^{[23
,24
]}

Vodenkova, Sona ^{[23
,24
]}

de Andrade, Vanessa Moraes ^{[25
]}

Sramkova, Monika ^{[26
]}

Gabelova, Alena ^{[26
]}

Collins, Andrew ^{[27
]}

Langie, Sabine A. S. ^{[12
,28
]}

机构：

[1] Univ Copenhagen, Sect Environm Hlth, Dept Publ Hlth, Copenhagen, Denmark

[2] Univ Navarra, Dept Pharmacol & Toxicol, Pamplona, Spain

[3] Navarra Inst Hlth Res, IdiSNA, Pamplona, Spain

[4] Univ Toulouse, INRAE, ENVT, Toxalim Res Ctr Food Toxicol,INP Purpan,UPS, Toulouse, France

[5] VITO Hlth, Mol, Belgium

[6] IRCCS San Raffaele Pisana, Unit Clin & Mol Epidemiol, Rome, Italy

[7] San Raffaele Univ, Dept Human Sci & Qual Life Promot, Rome, Italy

[8] Inst Med Res & Occupat Hlth, Mutagenesis Unit, Zagreb, Croatia

[9] Natl Inst Hlth Dr Ricardo Jorge, Environm Hlth Dept, Porto, Portugal

[10] Univ Porto, Inst Saude Publ, Epidemiol Res Unit EPIUnit, Porto, Portugal

[11] Norwegian Inst Air Res NILU, Dept Environm Chem, Hlth Effects Lab, Kjeller, Norway

[12] Maastricht Univ, Sch Nutr & Translat Res Metab NUTRIM, Dept Pharmacol & Toxicol, Maastricht, Netherlands

[13] Norwegian Inst Publ Hlth Skoyen, Dept Environm Hlth, Sect Mol Toxicol, Oslo, Norway

[14] Univ Florence, Sect Pharmacol & Toxicol, Dept Neurosci Psychol Pharmacol & Child Hlth NEUR, Florence, Italy

[15] Univ S Florida, Dept Cell Biol Microbiol & Mol Biol, Oxidat Stress Grp, Tampa, FL 33620 USA

[16] Univ Kaiserslautern, Dept Chem, Food Chem & Toxicol, Kaiserslautern, Germany

[17] Univ A Coruna, Fac Ciencias Educ, Dept Psicol, Ctr Invest Cient Avanzadas CICA,Grp DICOMOSA, Coruna, Spain

[18] AE CICA INIBIC, Inst Invest Biomed A Coruna INIBIC, Coruna, Spain

[19] Hacettepe Univ, Dept Toxicol, Fac Pharm, Ankara, Turkey

[20] Univ Milan, Dept Food Environm & Nutr Sci DeFENS, Div Human Nutr, Milan, Italy

[21] Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana, Slovenia

[22] Univ Wurzburg, Inst Pharmacol & Toxicol, Wurzburg, Germany

[23] Czech Acad Sci, Inst Expt Med, Prague, Czech Republic

[24] Charles Univ Prague, Med Fac Pilsen, Biomed Ctr, Prague, Czech Republic

[25] Univ Southern Santa Catarina, Lab Translat Biomed, UNESC, Criciuma, SC, Brazil

[26] Slovak Acad Sci, Biomed Res Ctr, Canc Res Inst, Dept Nanobiol, Bratislava, Slovakia

[27] Univ Oslo, Dept Nutr, Oslo, Norway

[28] Hasselt Univ, Ctr Environm Sci, Hasselt, Belgium

来源：

NATURE PROTOCOLS | 2020年 / 15卷 / 12期

关键词：

INTER-LABORATORY VARIATION; DNA-DAMAGE; ANIMAL-MODELS; CELLS; REPAIR; TIME; MIGRATION; OXIDATION; WHALES; BASE;

D O I：

10.1038/s41596-020-0398-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers. Here, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).

引用

页码：3817 / 3826

页数：10

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