DOSE-DEPENDENT INFLUENCE OF SHORT-TERM INTERMITTENT ETHANOL INTOXICATION ON CEREBRAL NEUROCHEMICAL CHANGES IN RATS DETECTED BY EX VIVO PROTON NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

被引：12

作者：

Lee, Do-Wan ^{[1
,2
]}

Nam, Yoon-Ki ^{[3
,4
]}

Kim, Tai-Kyung ^{[5
]}

Kim, Jae-Hwa ^{[6
]}

Kim, Sang-Young ^{[1
,2
]}

Min, Jung-Whan ^{[7
]}

Lee, Jung-Hoon ^{[1
,2
,8
]}

Kim, Hwi-Yool ^{[5
]}

Kim, Dai-Jin ^{[6
,9
]}

Choe, Bo-Young ^{[1
,2
]}

机构：

[1] Catholic Univ Korea, Dept Biomed Engn, Coll Med, Seoul 137701, South Korea

[2] Catholic Univ Korea, Res Inst Biomed Engn, Seoul 137701, South Korea

[3] Agilent Technol Korea Ltd, NMR Res Team, Seoul 135848, South Korea

[4] Agilent Technol Korea Ltd, Life Sci Grp, Seoul 135848, South Korea

[5] Konkuk Univ, Dept Vet Surg, Seoul 143701, South Korea

[6] Catholic Univ Korea, Coll Med, Dept Biomed Sci, Seoul 137701, South Korea

[7] Shingu Univ, Coll Korea, Dept Radiol Sci, Songnam 462743, Gyeonggi Do, South Korea

[8] Kyunghee Med Ctr, Dept Radiol, Seoul 130872, South Korea

[9] Catholic Univ Korea, Coll Med, Dept Psychiat, Seoul St Marys Hosp, Seoul 137701, South Korea

来源：

NEUROSCIENCE | 2014年 / 262卷

基金：

新加坡国家研究基金会;

关键词：

intermittent ethanol intoxication; brain; metabolites; frontal cortex; high-resolution spectra; GAMMA-AMINOBUTYRIC-ACID; IN-VIVO; LIPID-PEROXIDATION; BINGE DRINKING; BRAIN-DAMAGE; H-1-NMR SPECTROSCOPY; HEAVY DRINKING; ALCOHOL; CONSUMPTION; METABOLITES;

D O I：

10.1016/j.neuroscience.2013.12.061

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The aim of this study was to quantitatively assess the effects of short-term intermittent ethanol intoxication on cerebral metabolite changes among sham controls (CNTL), low-dose ethanol (LDE)-exposed, and high-dose ethanol (HDE)-exposed rats, which were determined with ex vivo high-resolution spectra. Eight-week-old male Wistar rats were divided into three groups. Twenty rats in the LDE (n = 10) and the HDE (n = 10) groups received ethanol doses of 1.5 and 2.5 g/kg, respectively, through oral gavage every 8 h for 4 days. At the end of the 4-day intermittent ethanol exposure, one-dimensional ex vivo 500-MHz H-1 nuclear magnetic resonance spectra were acquired from 30 samples of the frontal cortex region (from the three groups). Normalized total N-acetylaspartate (tNAA: NAA+ NAAG [N-acetyl-aspartyl-glutamate]), GABA, and glutathione (GSH) levels were significantly lower in the frontal cortex of the HDE-exposed rats than that of the LDE-exposed rats. Moreover, compared to the CNTL group, the LDE rats exhibited significantly higher normalized GABA levels. The six pairs of normalized metabolite levels were positively (+) or negatively (-) correlated in the rat frontal cortex as follows: tNAA and GABA (+), tNAA and aspartate (Asp) (+), myo-Inositol (mIns) and Asp (-), mIns and alanine (+), mIns and taurine (+), and mIns and tNAA (-). Our results suggested that short-term intermittent ethanol intoxication might result in neuronal degeneration and dysfunction, changes in the rate of GABA synthesis, and oxidative stress in the rat frontal cortex. Our ex vivo H-1 high-resolution magic angle spinning nuclear magnetic resonance spectroscopy results suggested some novel metabolic markers for the dose-dependent influence of short-term intermittent ethanol intoxication in the frontal cortex. (C) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.

引用

页码：107 / 117

页数：11

共 51 条

[1] The effect of ethanol on lipid peroxidation and glutathione level in the brain stem of rat [J].