Morphology and 18S rDNA gene sequence of Blepharisma sinuosum Sawaya, 1940 (Ciliophora: Heterotrichea) from Brazil

被引：14

作者：

Fernandes, Noemi Mendes ^{[1
]}

Pedroso Dias, Roberto Junio ^{[1
]}

Xavier Senra, Marcus Vinicius ^{[2
]}

Gomes Soares, Carlos Augusto ^{[2
]}

da Silva Neto, Inacio Domingos ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Dept Zool, Lab Protistol, BR-21941 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Dept Genet, Lab Genet Mol Eucariontes & Simbiontes, BR-21941 Rio De Janeiro, Brazil

来源：

EUROPEAN JOURNAL OF PROTISTOLOGY | 2013年 / 49卷 / 04期

关键词：

18S rDNA; Blepharisma; Brazil; Ciliophora; Macronucleus; Morphology; CILIATES PROTOZOA; PHYLOGENY; UNDULANS;

D O I：

10.1016/j.ejop.2013.04.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The morphology and morphometric data of seven populations of Blepharisma sinuosum from southeastern Brazil were investigated. The description is based on live observations, protargol impregnation, and scanning electron microscopy. Blepharisma sinuosum measures 75-255 mu m in length and 25-93 mu m in width and has a spindle-shaped body, pink color, a single contractile vacuole located at the posterior end, 50 adoral membranelles, a conspicuous paroral, 17-35 somatic kineties, a moniliform macronucleus with 2-7 connected nodules, and 3-20 micronuclei. Morphological comparisons with similar species were performed and suggest that B. americanum is the junior synonym of B. sinuosum. The 18S rDNA gene sequence of B. sinuosum was obtained and compared with that of other Blepharisma species. The length and GC content of the obtained sequence is 1652 bp and 47.03%, respectively, and has a very high structural similarity (99.9%) with the B. undulans sequence. The validity of the classification of Blepharisma species in morphonuclear subgenera is also discussed. (C) 2013 Elsevier GmbH. All rights reserved.

引用

页码：623 / 633

页数：11

共 26 条

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