Rice FLOURY ENDOSPERM10 encodes a pentatricopeptide repeat protein that is essential for the trans-splicing of mitochondrial nad1 intron 1 and endosperm development

被引：86

作者：

Wu, Mingming ^{[1
]}

Ren, Yulong ^{[2
]}

Cai, Maohong ^{[1
]}

Wang, Yunlong ^{[1
]}

Zhu, Shanshan ^{[2
]}

Zhu, Jianping ^{[1
]}

Hao, Yuanyuan ^{[1
]}

Teng, Xuan ^{[1
]}

Zhu, Xiaopin ^{[1
]}

Jing, Ruonan ^{[1
]}

Zhang, Huan ^{[1
]}

Zhong, Mingsheng ^{[1
]}

Wang, Yongfei ^{[1
]}

Lei, Cailin ^{[2
]}

Zhang, Xin ^{[2
]}

Guo, Xiuping ^{[2
]}

Cheng, Zhijun ^{[2
]}

Lin, Qibing ^{[2
]}

Wang, Jie ^{[2
]}

Jiang, Ling ^{[1
]}

Bao, Yiqun ^{[3
]}

Wang, Yihua ^{[1
]}

Wan, Jianmin ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Plant Gene Engn Res Ctr, Nanjing 210095, Jiangsu, Peoples R China

[2] Chinese Acad Agr Sci, Natl Key Facil Crop Resources & Genet Improvement, Beijing 100081, Peoples R China

[3] Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China

来源：

NEW PHYTOLOGIST | 2019年 / 223卷 / 02期

基金：

国家重点研发计划;

关键词：

aleurone layer; endosperm development; FLO10; intron splicing; mitochondria; nad1; pentatricopeptide repeat (PPR); rice; CYTOPLASMIC MALE-STERILITY; MESSENGER-RNA; SEED DEVELOPMENT; STARCH SYNTHESIS; TRANSCRIPTION FACTOR; PLANT MITOCHONDRIAL; ALTERNATIVE OXIDASE; PPR PROTEIN; GENE; COMPLEX;

D O I：

10.1111/nph.15814

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Endosperm, the major storage organ in cereal grains, determines grain yield and quality. Despite the fact that a role for P-type pentatricopeptide repeat (PPR) proteins in the regulation of endosperm development has emerged, molecular functions of many P-type PPR proteins remain obscure. Here, we report a rice endosperm defective mutant, floury endosperm10 (flo10), which developed smaller starch grains in starchy endosperm and abnormal cells in the aleurone layer. Map-based cloning and rescued experiments showed that FLO10 encodes a P-type PPR protein with 26 PPR motifs, which is localized to mitochondria. Loss of function of FLO10 affected the trans-splicing of the mitochondrial nad1 intron 1, which was accompanied by the increased accumulation of the nad1 exon 1 and exons 2-5 precursors. The failed formation of mature nad1 led to a dramatically decreased assembly and activity of complex I, reduced ATP production, and changed mitochondrial morphology. In addition, loss of function of FLO10 significantly induced an alternative respiratory pathway involving alternative oxidase. These results reveal that FLO10 plays an important role in the maintenance of mitochondrial function and endosperm development through its effect on the trans-splicing of the mitochondrial nad1 intron 1 in rice.

引用

页码：736 / 750

页数：15

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