Molecular Epidemiology and Virulence Features of Staphylococcus aureus Bloodstream Isolates in a Regional Burn Center in China, 2012-2016

被引:10
作者
Liu, Yang [1 ]
Du, Fang-ling [1 ]
Liu, Pan-pan [1 ]
Mei, Yan-fang [1 ]
Wan, La-gen [1 ]
Wei, Dan-dan [1 ]
Xu, Heng-yi [2 ]
Zhang, Wei [3 ]
机构
[1] Nanchang Univ, Dept Clin Microbiol, Affiliated Hosp 1, Nanchang, Jiangxi, Peoples R China
[2] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang, Jiangxi, Peoples R China
[3] Nanchang Univ, Dept Respirat, Affiliated Hosp 1, Yong Wai Zheng Jie 17, Nanchang 330006, Jiangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Staphylococcus aureus; molecular characteristics; burns; bloodstream infection; virulence; INFECTIONS; SUSCEPTIBILITY; BACTEREMIA; RESISTANCE; HOSPITALS; MORTALITY; CHILDREN;
D O I
10.1089/mdr.2017.0209
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Staphylococcus aureus is known to be a predominant pathogen causing bloodstream infection (BSI) from burn units. Our study aimed to perform the clinical epidemiological analysis and virulence features of S. aureus strains isolated from the burn patients with BSI from a burn center in southeastern China during 2012-2016. A collection of 112 S. aureus isolates causing BSI from burn center of a tertiary care hospital in China was carried out. Antimicrobial susceptibility testing was conducted in accordance with the Clinical and Laboratory Standards Institute (CLSI) guidelines. Toxin gene profiles, multilocus sequence typing, staphylococcal protein A (spa) typing, accessory gene regulator (agr) locus typing, staphylococcal cassette chromosome mec (SCCmec) typing, and dendrographic analysis were used to characterize and analyze these isolates. Of 112 S. aureus isolates, 52 (46.4%) were methicillin-resistant S. aureus (MRSA) and 60 (53.6%) were methicillin-susceptible S. aureus (MSSA). ST239-SCCmec III-t030-agr I was the major prevalent clone (26 from MRSA and 6 from MSSA), which was followed by ST239-SCCmec III-t037-agr I (12, 10.0%) and ST5-SCCmec II-t002-agr I (11, 9.2%). The genotyping results showed high genetic diversity in molecular characterization and toxin gene profiles of the strains. Carriage of tsst-1 was mainly associated with ST239-SCCmec III-t030-agr I and ST30-SCCmec IV-t062-agr III, whereas lukS/F-PV was distributed in different clones. In conclusion, ST239-SCCmec III-t030-agr I is the commonest clone causing BSI among burn patients in eastern regions of China. In contrast to MRSA, polyclonality was statistically significantly higher among MSSA isolated from burn patients with BSI.
引用
收藏
页码:1354 / 1360
页数:7
相关论文
共 36 条
  • [31] Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States
    Tenover, FC
    McDougal, LK
    Goering, RV
    Killgore, G
    Projan, SJ
    Patel, JB
    Dunman, PM
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (01) : 108 - 118
  • [32] Molecular Characterization of Community Acquired Staphylococcus aureus Bacteremia in Young Children in Southern Mozambique, 2001-2009
    Vubil, Delfino
    Garrine, Marcelino
    Ruffing, Ulla
    Acacio, Sozinho
    Sigaque, Betuel
    Alonso, Pedro L.
    von Mueller, Lutz
    Herrmann, Mathias
    Mandomando, Inacio
    [J]. FRONTIERS IN MICROBIOLOGY, 2017, 8
  • [33] Wang Wen-Kui, 2009, Zhonghua Shao Shang Za Zhi, V25, P94
  • [34] Community-acquired methicillin-resistant Staphylococcus aureus: what do we need to know?
    Witte, W.
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2009, 15 : 17 - 25
  • [35] Prevalence and Molecular Epidemiology of Staphylococcus aureus among Residents of Seven Nursing Homes in Shanghai
    Zhang, Ji
    Gu, Fei-Fei
    Zhao, Sheng-Yuan
    Xiao, Shu-Zhen
    Wang, Yan-Chun
    Guo, Xiao-Kui
    Ni, Yu-Xing
    Han, Li-Zhong
    [J]. PLOS ONE, 2015, 10 (09):
  • [36] Characterization of Community Acquired Staphylococcus aureus Associated with Skin and Soft Tissue Infection in Beijing: High Prevalence of PVL+ ST398
    Zhao, Chunjiang
    Liu, Yingmei
    Zhao, Mingze
    Liu, Yali
    Yu, Yong
    Chen, Hongbin
    Sun, Qiuning
    Chen, Huawei
    Jiang, Wei
    Liu, Yudong
    Han, Shaomei
    Xu, Yingchun
    Chen, Minjun
    Cao, Bin
    Wang, Hui
    [J]. PLOS ONE, 2012, 7 (06):