Long-term, low-maintenance storage of Acanthamoeba strains

被引：10
作者：
Lorenzo-Morales, Jacob
Maciver, Sutherland K.
机构：
[1] Univ Edinburgh, Coll Med, Sch Biomed Sci, Edinburgh EH8 9XD, Midlothian, Scotland
[2] Univ La Laguna, Univ Inst Trop Dis & Publ Hlth Canary Isl, E-38207 San Cristobal la Laguna, Canary Islands, Spain
来源：
PARASITOLOGY RESEARCH | 2006年 / 99卷 / 06期
关键词：
PRESERVATION; CASTELLANII; NAEGLERIA; AMEBAS; CYSTS;
D O I：
10.1007/s00436-006-0212-1
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A simple, low cost method is described for the long-term storage of the potential human pathogen Acanthamoeba. Amoebae are placed in dried, sterile clay pellets within which they form cysts that remain viable and pure for at least 5 years. This method compares favorably with other storage methods and requires no specialist equipment. We have demonstrated that amoeba stored by this method retain both their pathogenic potential and their small subunit ribosomal DNA sequence type.
引用
收藏
页码：743 / 745
页数：3
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