25-Hydroxycholesterol promotes migration and invasion of lung adenocarcinoma cells

被引：42

作者：

Chen, Li ^{[1
]}

Zhang, Lishan ^{[2
]}

Xian, Guozhe ^{[3
]}

Lv, Yinping ^{[4
]}

Lin, Yanliang ^{[1
]}

Wang, Yibing ^{[5
]}

机构：

[1] Shandong Univ, Shandong Prov Hosp, Ctr Lab, Jinan 250021, Peoples R China

[2] Shandong Univ, Shandong Prov Hosp, Dept Hand & Foot Surg, Jinan 250021, Peoples R China

[3] Shandong Univ, Shandong Prov Hosp, Dept Hepatobiliary Surg, Jinan 250021, Peoples R China

[4] Dept Med Engn, Jinan 250021, Peoples R China

[5] Shandong Univ, Shandong Prov Hosp, Dept Burn & Plast Surg, Jinan 250021, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 484卷 / 04期

基金：

中国国家自然科学基金;

关键词：

25-Hydroxycholesterol; Lung adenocarcinoma; Migration; Invasion; LXR; IL-1; beta; BREAST-CANCER CELLS; RECEPTOR ACTIVATION; CHOLESTEROL; MACROPHAGES; METASTASIS; INTERFERON; OXYSTEROLS; RESPONSES; PATHWAY;

D O I：

10.1016/j.bbrc.2017.02.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

25-hydroxycholesterol (25-HC) is enzymatically produced by cholesterol 25-hydorxylase in various organs and is involved in many processes, including lipid metabolism, inflammation and the immune response. However, the role of 25-HC in the migration and invasion of lung adenocarcinoma (ADC) cells remains largely unknown. In this study, we demonstrated that 0.1 mu M 25-HC promoted ADC cell migration and invasion without affecting cell proliferation, especially after coculture with THP1-derived macrophages. Further investigation showed that 0.1 mu M 25-HC significantly stimulated interleukin-1 beta (IL-1 beta) secretion in a coculture system and increased the expression of LXR and Snail. IL-1 beta also mimicked the effect of 25-HC. LXR knockdown notably blocked the 25-HC-induced Snail expression, migration and invasion in both the monoculture system and the coculture system, but it did not impact the effect of IL-1 beta, which suggested that IL-1 beta functioned in an LXR-independent manner. These results suggested that 25-HC promoted ADC cell migration and invasion in an LXR-dependent manner in the monoculture system but that in the coculture system, the 25-HC-induced IL-1 beta secretion enhanced the effect of 25-HC in an LXR-independent manner. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：857 / 863

页数：7

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