Expression of and cytokine activation by Escherichia coli curli fibers in human sepsis

被引:154
作者
Bian, Z
Brauner, A
Li, YH
Normark, S
机构
[1] Karolinska Hosp, Ctr Microbiol & Tumor Biol, S-10401 Stockholm, Sweden
[2] Karolinska Hosp, Dept Clin Microbiol, S-10401 Stockholm, Sweden
关键词
D O I
10.1086/315233
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Curli organelles are expressed by commensal Escherichia coli K12 and by Salmonella typhimurium at temperatures <37 degrees C, which bind serum proteins and activate the contact-phase system in vitro. This study demonstrates, by means of an anti-CsgA (curli major subunit) antibody that a significant fraction of E. coli isolates (24 of 46) from human blood cultures produce curli at 37 degrees C in vitro. Serum samples from 12 convalescent patients with sepsis, but not serum from healthy controls, contained antibodies against CsgA (n = 12). This study further demonstrates that a curli-expressing E: coli strain and a noncurliated mutant secreting soluble CsgA induce significantly (P <.05) higher levels of proinflammatory cytokines (tumor necrosis factor-alpha, interleukin [IL]-6, and IL-8) in human macrophages differentiated from THP-1 cells. These data, therefore, provide direct evidence that curli are expressed in vivo in human sepsis and suggest a possible role for curli and CsgA in the induction of proinflammatory cytokines during E. coli sepsis.
引用
收藏
页码:602 / 612
页数:11
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