The role of bone marrow derived-mesenchymal stem cells in attenuation of kidney function in rats with diabetic nephropathy

被引：58

作者：

Aziz, Mohamed Talaat Abdel ^{[1
]}

Wassef, Mohamed Abdel Aziz ^{[1
]}

Ahmed, Hanan Hosni ^{[1
]}

Rashed, Laila ^{[1
]}

Mahfouz, Soheir ^{[2
]}

Aly, Mayssa Ibrahim ^{[3
]}

Hussein, Rania Elsayed ^{[1
]}

Abdelaziz, Mai ^{[1
]}

机构：

[1] Cairo Univ, Fac Med, Dept Med Biochem, Unit Biochem & Mol Biol, Cairo, Egypt

[2] Cairo Univ, Fac Med, Dept Pathol, Cairo, Egypt

[3] Cairo Univ, Fac Med, Dept Internal Med, Cairo, Egypt

来源：

DIABETOLOGY & METABOLIC SYNDROME | 2014年 / 6卷

关键词：

Stem cells therapy; Mesenchymal stem cells; Diabetic nephropathy; GLYCATION END-PRODUCTS; TUMOR-NECROSIS-FACTOR; NITRIC-OXIDE SYNTHASE; FACTOR-ALPHA; TNF-ALPHA; RECEPTOR; EXPRESSION; GLOMERULOSCLEROSIS; TRANSPLANTATION; INFLAMMATION;

D O I：

10.1186/1758-5996-6-34

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Stem cell therapy holds a great promise for the repair of injured tissues and organs, including the kidney. We studied the effect of mesenchymal stem cells (MSC) on experimental diabetic nephropathy (DN) in rats and the possible paracrine signals that mediate their action. Materials and methods: Rats were divided into controls, DN rats, DN rats receiving MSCs. MSCs were given in a dose of (10(6)cells) by intravenous injection. After 4 weeks, 24 h urinary albumin, serum urea and creatinine concentrations, transforming growth factor beta (TGF beta), tumor necrosis factor alpha (TNF alpha), B-cell lymphoma 2 (bcl2) and Bax gene expression and vascular endothelial growth factor (VEGF) were assessed. Histopathology staining was performed. Results: MSC therapy significantly improved 24 h urinary albumin, serum urea and creatinine concentrations, increased angiogenic growth factor VEGF, and anti-apoptotic protein bcl2 while decreased the pro-inflammatory TNF-alpha, fibrogenic growth factor TGF beta, and pro-apoptotic protein Bax. The histopathology examination showed patchy areas of minimal necrosis and degeneration in renal tubules.

引用

页数：10

共 50 条

[1]

Abdel Aziz M.T., 2011, J STEM CELL RES THER, V1, P2

[2]

Abdel Aziz MT, 2011, STEM CELL STUDIES, V1, pE11

[3] Immunomodulation by mesenchymal stem cells - A potential therapeutic strategy for type 1 diabetes [J].

Abdi, Reza ;

Fiorina, Paolo ;

Adra, Chaker N. ;

Atkinson, Mark ;

Sayegh, Mohamed H. .

DIABETES, 2008, 57 (07) :1759-1767

[4] In search of adult renal stem cells [J].

Anglani, F ;

Forino, M ;

Del Prete, D ;

Tosetto, E ;

Torregrossa, R ;

D'Angelo, A .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2004, 8 (04) :474-487

[5] Therapeutic potential of bone marrow-derived mesenchymal stem cells on experimental liver fibrosis [J].

Aziz, M. T. Abdel ;

Atta, H. M. ;

Mahfouz, S. ;

Fouad, H. H. ;

Roshdy, N. K. ;

Ahmed, H. H. ;

Rashed, L. A. ;

Sabry, D. ;

Hassouna, A. A. ;

Hasan, N. M. .

CLINICAL BIOCHEMISTRY, 2007, 40 (12) :893-899

[6] TUMOR NECROSIS FACTOR STIMULATES PROSTAGLANDIN PRODUCTION AND CYCLIC-AMP LEVELS IN RAT CULTURED MESANGIAL CELLS [J].

BAUD, L ;

PEREZ, J ;

FRIEDLANDER, G ;

ARDAILLOU, R .

FEBS LETTERS, 1988, 239 (01) :50-54

[7]

BERTANI T, 1989, AM J PATHOL, V134, P419

[8] Tumor necrosis factor-α promotes macrophage-induced vascular smooth muscle cell apoptosis by direct and autocrine mechanisms [J].

Boyle, JJ ;

Weissberg, PL ;

Bennett, MR .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2003, 23 (09) :1553-1558

[9]

BRETT J, 1993, AM J PATHOL, V143, P1699

[10] Long-term treatment of focal segmental glomerulosclerosis in children with cyclosporine given as a single daily dose [J].

Chishti, AS ;

Sorof, JM ;

Brewer, ED ;

Kale, AS .

AMERICAN JOURNAL OF KIDNEY DISEASES, 2001, 38 (04) :754-760

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