Disruption of MiaA provides insights into the regulation of phenazine biosynthesis under suboptimal growth conditions in Pseudomonas chlororaphis 30-84

被引:10
|
作者
Yu, Jun Myoung [1 ]
Wang, Dongping [2 ]
Pierson, Leland S., III [1 ]
Pierson, Elizabeth A. [1 ,3 ]
机构
[1] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77943 USA
[2] Los Alamos Natl Lab, Earth & Environm Sci, Los Alamos, NM 87544 USA
[3] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77943 USA
来源
MICROBIOLOGY-SGM | 2017年 / 163卷 / 01期
基金
美国农业部;
关键词
MiaA; tRNA modification; gene regulation; Pseudomonas chlororaphis; biological control; TRANSFER-RNA MODIFICATION; ESCHERICHIA-COLI K-12; AUREOFACIENS STRAIN 30-84; QUORUM-SENSING SYSTEM; ANTIBIOTIC BIOSYNTHESIS; BIOFILM FORMATION; FLUORESCENT PSEUDOMONADS; SALMONELLA-TYPHIMURIUM; MODIFICATION ENZYME; BIOLOGICAL-CONTROL;
D O I
10.1099/mic.0.000409
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Many products of secondary metabolism are activated by quorum sensing (QS), yet even at cell densities sufficient for QS, their production may be repressed under suboptimal growth conditions via mechanisms that still require elucidation. For many beneficial plant-associated bacteria, secondary metabolites such as phenazines are important for their competitive survival and plant-protective activities. Previous work established that phenazine biosynthesis in Pseudomonas chlororaphis 30-84 is regulated by the PhzR/PhzI QS system, which in turn is regulated by transcriptional regulator Pip, two-component system RpeA/RpeB and stationary phase/stress sigma factor RpoS. Disruption of MiaA, a tRNA modification enzyme, altered primary metabolism and growth leading to widespread effects on secondary metabolism, including reduced phenazine production and oxidative stress tolerance. Thus, the miaA mutant provided the opportunity to examine the regulation of phenazine production in response to altered metabolism and growth or stress tolerance. Despite the importance of MiaA for translation efficiency, the most significant effect of miaA disruption on phenazine production was the reduction in the transcription of phzR, phzI and pip, whereas neither the transcription nor translation of RpeB, a transcriptional regulator of pip, was affected. Constitutive expression of rpeB or pip in the miaA mutant completely restored phenazine production, but it resulted in further growth impairment. Constitutive expression of RpoS alleviated sensitivity to oxidative stress resulting from RpoS translation inefficiency in the miaA mutant, but it did not restore phenazine production. Our results support the model that cells curtail phenazine biosynthesis under suboptimal growth conditions via RpeB/Pip-mediated regulation of QS.
引用
收藏
页码:94 / 108
页数:15
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