Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass

被引：85

作者：

Wang, Le ^{[1
]}

Wan, Zi Yi ^{[1
]}

Bai, Bin ^{[1
]}

Huang, Shu Qing ^{[1
]}

Chua, Elaine ^{[1
]}

Lee, May ^{[1
]}

Pang, Hong Yan ^{[1
]}

Wen, Yan Fei ^{[1
]}

Liu, Peng ^{[1
]}

Liu, Feng ^{[1
]}

Sun, Fei ^{[1
]}

Lin, Grace ^{[1
]}

Ye, Bao Qing ^{[1
]}

Yue, Gen Hua ^{[1
,2
,3
]}

机构：

[1] Natl Univ Singapore, Temasek Life Sci Lab, Res Link 1, Singapore 117604, Singapore

[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

[3] Nanyang Technol Univ, Sch Biol Sci, Singapore 637551, Singapore

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

新加坡国家研究基金会;

关键词：

HIGH-RESOLUTION LINKAGE; LATES-CALCARIFER; SNP DISCOVERY; GENETIC-MAP; DE-NOVO; GENOME; IDENTIFICATION; TRANSCRIPTOME; RESISTANCE; TRAITS;

D O I：

10.1038/srep16358

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A high-density genetic map is essential for comparative genomic studies and fine mapping of QTL, and can also facilitate genome sequence assembly. Here, a high density genetic map of Asian seabass was constructed with 3321 SNPs generated by sequencing 144 individuals in a F-2 family. The length of the map was 1577.67 cM with an average marker interval of 0.52 cM. A high level of genomic synteny among Asian seabass, European seabass, Nile tilapia and stickleback was detected. Using this map, one genome-wide significant and five suggestive QTL for growth traits were detected in six linkage groups (i.e. LG4, LG5, LG11, LG13, LG14 and LG15). These QTL explained 10.5-16.0% of phenotypic variance. A candidate gene, ACOX1 within the significant QTL on LG5 was identified. The gene was differentially expressed between fast- and slow-growing Asian seabass. The high-density SNP-based map provides an important tool for fine mapping QTL in molecular breeding and comparative genome analysis.

引用

页数：10

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