Optimizing RNA extraction yield from whole blood for microarray gene expression analysis

被引：29
|
作者：
Wang, J
Robinson, JF
Khan, HMR
Carter, DE
McKinney, J
Miskie, BA
Hegele, RA
机构：
[1] John P Robarts Res Inst, Blackburn Cardiovasc Genet Lab, London, ON N6A 5K8, Canada
[2] John P Robarts Res Inst, Vasc Biol Res Grp, London, ON N6A 5K8, Canada
[3] John P Robarts Res Inst, London Reg Genom Ctr, London, ON N6A 5K8, Canada
[4] Univ Western Ontario, London, ON N6A 5K8, Canada
来源：
CLINICAL BIOCHEMISTRY | 2004年 / 37卷 / 09期
基金：
加拿大健康研究院;
关键词：
molecular diagnosis; gene expression; immunology epidemiology; metabolic disease;
D O I：
10.1016/j.clinbiochem.2004.03.013
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: Microarray analysis of gene expression profiles of blood leukocytes has many potential clinical and research applications. Design and methods: We used the PAXgene Blood RNA System to prepare RNA from the whole blood of normal volunteers using two incubation times followed by gene expression profiling using the Affymetrix HU133A GeneChip. Conclusions: Longer incubation gave a significantly higher RNA yield and samples that were satisfactory for microarray analysis, with excellent pairwise correlations between replicates. (C) 2004 The Canadian Society of Clinical Chemists. All rights reserved.
引用
收藏
页码：741 / 744
页数：4
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