Simultaneous determination of four tobacco-specific N-nitrosamines (TSNA) in human urine

被引：48
|
作者：
Kavvadias, Dominique [1 ]
Scherer, Gerhard [1 ]
Urban, Michael [1 ]
Cheung, Francis [2 ]
Errington, Graham [2 ]
Shepperd, Jim [2 ]
McEwan, Mike [2 ]
机构：
[1] Analyt Biolog Forsch Labor GmbH, D-80336 Munich, Germany
[2] Res & Dev Grp, Southampton SO15 8TL, Hants, England
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 11-12期
关键词：
Tobacco-specific N-nitrosamines (TSNA); 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK); 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL); N-nitrosonornicotine (NNN); N-nitrosoanabasine (NAB); N-nitrosoanatabine (NAT); Biological monitoring; Tobacco smoking; Human urine; PATAS MONKEY; METABOLITES; 4-(METHYLNITROSAMINO)-1-(3-PYRIDYL)-1-BUTANONE; PHARMACOKINETICS; CANCER; SMOKE;
D O I：
10.1016/j.jchromb.2009.03.009
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Tobacco-specific N-nitrosamines (TSNA) include 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosonornicotine (NNN), N-nitrosoanabasine (NAB) and N-nitrosoanatabine (NAT). TSNA are suggested to play an important role in tobacco smoke carcinogenesis. We have developed and validated an LC-MS/MS method for the determination of total (free and conjugated)TSNA in human urine. The limits of detection (LOD) were 2.0, 0.8, 1.1 and 0.7 pg/ml for NNAL, NNN, NAB and NAT, respectively. Smokers were found to have significantly higher levels of TSNA in their urine than nonsmokers. In conclusion, the newly developed method is suitable for assessing the tobacco use-related exposure to NNK, NNN, NAB and NAT. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：1185 / 1192
页数：8
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