Simultaneous determination of four tobacco-specific N-nitrosamines (TSNA) in human urine

被引：48
|
作者：
Kavvadias, Dominique [1 ]
Scherer, Gerhard [1 ]
Urban, Michael [1 ]
Cheung, Francis [2 ]
Errington, Graham [2 ]
Shepperd, Jim [2 ]
McEwan, Mike [2 ]
机构：
[1] Analyt Biolog Forsch Labor GmbH, D-80336 Munich, Germany
[2] Res & Dev Grp, Southampton SO15 8TL, Hants, England
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 11-12期
关键词：
Tobacco-specific N-nitrosamines (TSNA); 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK); 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL); N-nitrosonornicotine (NNN); N-nitrosoanabasine (NAB); N-nitrosoanatabine (NAT); Biological monitoring; Tobacco smoking; Human urine; PATAS MONKEY; METABOLITES; 4-(METHYLNITROSAMINO)-1-(3-PYRIDYL)-1-BUTANONE; PHARMACOKINETICS; CANCER; SMOKE;
D O I：
10.1016/j.jchromb.2009.03.009
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Tobacco-specific N-nitrosamines (TSNA) include 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosonornicotine (NNN), N-nitrosoanabasine (NAB) and N-nitrosoanatabine (NAT). TSNA are suggested to play an important role in tobacco smoke carcinogenesis. We have developed and validated an LC-MS/MS method for the determination of total (free and conjugated)TSNA in human urine. The limits of detection (LOD) were 2.0, 0.8, 1.1 and 0.7 pg/ml for NNAL, NNN, NAB and NAT, respectively. Smokers were found to have significantly higher levels of TSNA in their urine than nonsmokers. In conclusion, the newly developed method is suitable for assessing the tobacco use-related exposure to NNK, NNN, NAB and NAT. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：1185 / 1192
页数：8
相关论文
共 50 条
  • [1] TOBACCO-SPECIFIC N-NITROSAMINES (TSNA) - INTRODUCTION
    HOFFMANN, D
    SPIEGELHALDER, B
    CRITICAL REVIEWS IN TOXICOLOGY, 1991, 21 (04) : U235 - U235
  • [2] TOBACCO-SPECIFIC N-NITROSAMINES (TSNA) - CONCLUDING REMARKS
    HOFFMANN, D
    CRITICAL REVIEWS IN TOXICOLOGY, 1991, 21 (04) : 312 - 313
  • [3] Determination of tobacco-specific N-nitrosamines in urine of smokers and non-smokers
    Kavvadias, Dominique
    Scherer, Gerhard
    Cheung, Francis
    Errington, Graham
    Shepperd, Jim
    McEwan, Mike
    BIOMARKERS, 2009, 14 (08) : 547 - 553
  • [4] PHARMACOKINETICS OF TOBACCO-SPECIFIC N-NITROSAMINES
    ADAMS, JD
    LAVOIE, EJ
    ODONNELL, M
    HOFFMANN, D
    IARC SCIENTIFIC PUBLICATIONS, 1984, (57) : 779 - 785
  • [5] NASAL CAVITY RHABDOMYOSARCOMA (NCR) IN RATS TREATED WITH TOBACCO-SPECIFIC N-NITROSAMINES (TSNA)
    RIVENSON, A
    MU, B
    CASTONGUAY, A
    ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1984, 435 (DEC) : 487 - 489
  • [6] TOBACCO-SPECIFIC N-NITROSAMINES [TSNA] IN GREEN MATURE AND PROCESSED TOBACCO-LEAVES FROM INDIA
    BHIDE, SV
    NAIR, J
    MARU, GB
    NAIR, UJ
    RAO, BVK
    CHAKRABORTY, MK
    BRUNNEMANN, KD
    BEITRAGE ZUR TABAKFORSCHUNG INTERNATIONAL, 1987, 14 (01): : 29 - 32
  • [7] CARCINOGENICITY OF TOBACCO-SPECIFIC N-NITROSAMINES (TSNA) - THE ROLE OF THE VASCULAR NETWORK IN THE SELECTION OF TARGET ORGANS
    RIVENSON, A
    HECHT, SS
    HOFFMANN, D
    CRITICAL REVIEWS IN TOXICOLOGY, 1991, 21 (04) : 255 - 264
  • [8] Formation and analysis of tobacco-specific N-nitrosamines
    Brunnemann, KD
    Prokopczyk, B
    Djordjevic, MV
    Hoffmann, D
    CRITICAL REVIEWS IN TOXICOLOGY, 1996, 26 (02) : 121 - 137
  • [9] TOBACCO-SPECIFIC N-NITROSAMINES IN DRY SNUFF
    ADAMS, JD
    OWENSTUCCIARONE, P
    HOFFMANN, D
    FOOD AND CHEMICAL TOXICOLOGY, 1987, 25 (03) : 245 - 246
  • [10] SUPERCRITICAL FLUID EXTRACTION IN THE DETERMINATION OF TOBACCO-SPECIFIC N-NITROSAMINES IN SMOKELESS TOBACCO
    PROKOPCZYK, B
    HOFFMANN, D
    COX, JE
    DJORDJEVIC, MV
    BRUNNEMANN, KD
    CHEMICAL RESEARCH IN TOXICOLOGY, 1992, 5 (03) : 336 - 340
12345