T-cell clones in human trichinellosis: Evidence for a mixed Th1/Th2 response

被引：26

作者：

Della Bella, C. ^{[1
]}

Benagiano, M. ^{[1
]}

De Gennaro, M. ^{[2
]}

Gomez-Morales, M. A. ^{[3
]}

Ludovisi, A. ^{[3
]}

D'Elios, S. ^{[1
]}

Luchi, S. ^{[2
]}

Pozio, E. ^{[3
]}

D'Elios, M. M. ^{[1
]}

Bruschi, F. ^{[4
,5
]}

机构：

[1] Univ Florence, Dept Expt & Clin Med, Florence, Italy

[2] Osped Lucca, Lucca, Italy

[3] Ist Super Sanita, Dept Infect Parasit & Immune Mediated Dis, Rome, Italy

[4] Univ Pisa, Dept Translat Res, NTMS, Pisa, Italy

[5] Baylor Coll Med, Natl Sch Trop Med, Houston, TX 77030 USA

来源：

PARASITE IMMUNOLOGY | 2017年 / 39卷 / 03期

关键词：

eosinophilia; human trichinellosis; IgE; T-cell clones; Th1/Th2 mixed response; TRICHINELLA-SPIRALIS; HELICOBACTER-PYLORI; CYSTIC ECHINOCOCCOSIS; CYTOKINE PRODUCTION; IN-VITRO; ANTIGENS; INFLAMMATION; INFECTION; PROFILE; LINES;

D O I：

10.1111/pim.12412

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In humans, studies on the cellular immune response against Trichinella are scarce. Aim of this study was to characterize the cytokine profile of T cells specific for Trichinella britovi in trichinellosis patients. Peripheral blood mononuclear cells (PBMC) were obtained from five patients involved in a trichinellosis outbreak caused by T. britovi, which occurred in 2013 in Tuscany (Italy). All the patients resulted positive for Trichinella-specific IgG, IgE and presented eosinophilia. T cells were investigated for their proliferation to excretory/secretory antigens from Trichinella spiralis muscle larvae (TsES) and for their cytokine profile. A total of 284 CD4+ and 42 CD8+ T-cell clones were obtained from the TsES-specific T-cell lines from PBMC. All T-cell clones proliferated in response to mitogen. Of the 284 CD4+ T-cell clones generated from TsES-specific T-cell lines, 135 (47%) proliferated significantly to TsES; 26% CD8+ T-cell clones showed proliferation to TsES. In the series of the 135 TsES-specific CD4+ clones, 51% expressed a Th2 profile, 30% a Th0 and 19% Th1. In the series of the 11 TsES-specific CD8+ T-cell clones, 18% were Tc2, 45% Tc0 and 36% Tc1. In human trichinellosis, the cellular immune response is, during the chronic phase, mixed Th1/Th2.

引用

页数：6

共 30 条

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