Direct determination of vibrational density of states change on ligand binding to a protein

被引：108
|
作者：
Balog, E
Becker, T
Oettl, M
Lechner, R
Daniel, R
Finney, J
Smith, JC
机构：
[1] Heidelberg Univ, IWR, D-69120 Heidelberg, Germany
[2] Univ Waikato, Dept Biol Sci, Hamilton, New Zealand
[3] BENSC, Hahn Meitner Inst Berlin, D-14109 Berlin, Germany
[4] UCL, Dept Phys & Astron, London WC1E 6BT, England
来源：
PHYSICAL REVIEW LETTERS | 2004年 / 93卷 / 02期
关键词：
D O I：
10.1103/PhysRevLett.93.028103
中图分类号：
O4 [物理学];
学科分类号：
0702 ;
摘要：
The change in the vibrational density of states of a protein (dihydrofolate reductase) on binding a ligand (methotrexate) is determined using inelastic neutron scattering. The vibrations of the complex soften significantly relative to the unbound protein. The resulting free-energy change, which is directly determined by the density of states change, is found to contribute significantly to the binding equilibrium.
引用
收藏
页码：028103 / 1
页数：4
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