Comparative study of Pd@Pt nanozyme improved colorimetric N-ELISA for the paper-output portable detection of Staphylococcus aureus

被引：39

作者：

Wang, Xin ^{[1
]}

Zhang, Min ^{[1
]}

Pang, Xiaoxu ^{[1
]}

Huang, Kunlun ^{[1
,2
]}

Yao, Zhiyi ^{[1
,2
]}

Mei, Xiaohong ^{[1
,2
]}

Cheng, Nan ^{[1
,2
]}

机构：

[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Lab Food Qual & Safety, Beijing 100083, Peoples R China

[2] Minist Agr, Key Lab Safety Assessment Genet Modified Organism, Beijing 100083, Peoples R China

来源：

TALANTA | 2022年 / 247卷

关键词：

Staphylococcus aureus; Pd@Pt nanozyme; Immunoassay; N-ELISA; NANOPARTICLES; IMMUNOSENSOR; IMMUNOASSAY; PEROXIDASE; ENZYME;

D O I：

10.1016/j.talanta.2022.123503

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Staphylococcus aureus is a frequent pathogenic bacterium that has a significant detrimental influence on the health of the human body. Therefore, developing a practical and portable detection platform is critical to ensuring food safety. Nanozymes are a kind of engineered nanomaterials with superior enzyme-like activities, providing infinite possibilities for the development of highly sensitive analytical assays. In this study, mesoporous core-shell palladium@platinum (Pd@Pt) nanozymes were synthesized and then applied as a signal amplifier in Staphylococcus aureus colorimetric immunoassay. At the same time, a careful comparative study of the catalytic performance with natural horseradish peroxidase (HRP), Pd@Pt nanozymes and their complexes Pd@Pt-HRP (HRP coupling with Pd@Pt nanozymes) were firstly performed, as well as clever using a colorimeter to achieve portable signal output. Pd@Pt-HRP bioprobes enable remarkable peroxidase-like catalytic activity, resulting in the highest sensitivity with a limit of detection (LOD) improved from 1 x 10(5) to 1 x 10(3) CFU/mL. In all, this work conducted a valuable comparative study and presented an improved strategy for the portable detection of pathogenic bacteria, which was expected to be used in a wide range of applications in food safety regulation and biomedicals arenas.

引用

页数：7

共 32 条

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