Enhanced Performance of Rhizopus oryzae Lipase by Reasonable Immobilization on Magnetic Nanoparticles and Its Application in Synthesis 1,3-Diacyglycerol

被引：30

作者：

Zhao, Jiong-feng ^{[1
]}

Lin, Jian-ping ^{[1
]}

Yang, Li-rong ^{[1
]}

Wu, Mian-bin ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Chem & Biol Engn, Key Lab Biomass Chem Engn, Minist Educ, Hangzhou 310027, Zhejiang, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2019年 / 188卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Nano-sized Fe3O4; Rhizopus oryzae lipase; Interface activated; Immobilization; 1; 3-diacyglycerols; MILK FAT SUBSTITUTES; INTERFACIAL ACTIVATION; LECITASE(R) ULTRA; ENZYME-ACTIVITY; STABILITY; DIACYLGLYCEROLS; ESTERIFICATION; GLYCEROLYSIS; BIOCATALYST; SELECTIVITY;

D O I：

10.1007/s12010-018-02947-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nano-sized Fe3O4 was synthesized by chemical co-precipitation and subsequently modified with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde to introduce aldehyde group on its surface. With the help of interface activation by adding sucrose esters-11 as surfactant, lipase from Rhizopus oryzae was successfully immobilized onto the carrier with great enhancement of activity. The hydrolysis activity of immobilized enzyme were 9.16 times and 31.6 times of free enzyme when p-nitrophenol butyrate and p-nitrophenol palmitate were used as substrates. The thermo-stability of immobilized enzyme was also enhanced compared to free enzyme. The immobilized enzyme was successfully applied in synthesis of 1,3-diacyglycerols (1,3-DAG). The specific esterification activity of immobilized enzyme was about 1.5 times of the free enzyme. The immobilized enzyme showed good region-selectivity towards 1,3-diacyglycerols and retained nearly 80% of its activity after reused for 60 times, revealing a good industrial application prospect.

引用

页码：677 / 689

页数：13

共 49 条

[1] Immobilisation and application of lipases in organic media [J].

Adlercreutz, Patrick .

CHEMICAL SOCIETY REVIEWS, 2013, 42 (15) :6406-6436

[2] Site-specific immobilization of genetically engineered variants of Candida antarctica lipase B [J].

Blank, Kerstin ;

Morfill, Julia ;

Gaub, Hermann E. .

CHEMBIOCHEM, 2006, 7 (09) :1349-1351

[3] Synthesis and Properties of Magnetite-Poly(aminopropylsiloxane) Composites [J].

Bondar', L. A. ;

Abramov, N. V. ;

Mishchenko, V. N. ;

Gorbik, P. P. .

COLLOID JOURNAL, 2010, 72 (01) :1-5

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] Catalytic biofunctional membranes containing site-specifically immobilized enzyme arrays: a review [J].

Butterfield, DA ;

Bhattacharyya, D ;

Daunert, S ;

Bachas, L .

JOURNAL OF MEMBRANE SCIENCE, 2001, 181 (01) :29-37

[6] Facile synthesis and magnetic properties of monodisperse Fe3O4/silica nanocomposite microspheres with embedded structures via a direct solution-based route [J].

Chen, Lingyun ;

Xu, Zhengxiong ;

Dai, Hong ;

Zhang, Shengtao .

JOURNAL OF ALLOYS AND COMPOUNDS, 2010, 497 (1-2) :221-227

[7] CATALYSIS AT THE INTERFACE - THE ANATOMY OF A CONFORMATIONAL CHANGE IN A TRIGLYCERIDE LIPASE [J].

DEREWENDA, U ;

BRZOZOWSKI, AM ;

LAWSON, DM ;

DEREWENDA, ZS .

BIOCHEMISTRY, 1992, 31 (05) :1532-1541

[8] Production of structured triacylglycerols rich in palmitic acid at sn-2 position and oleic acid at sn-1,3 positions as human milk fat substitutes by enzymatic acidolysis [J].

Esteban, Luis ;

Jimenez, Maria J. ;

Hita, Estrella ;

Gonzalez, Pedro A. ;

Martin, Lorena ;

Robles, Alfonso .

BIOCHEMICAL ENGINEERING JOURNAL, 2011, 54 (01) :62-69

[9] MECHANISMS FOR OXIDATION OF FE3O4 [J].

FEITKNECHT, W ;

GALLAGHE.FJ .

NATURE, 1970, 228 (5271) :548-+

[10] Comparison of the biochemical properties of a recombinant lipase extract from Rhizopus oryzae expressed in Pichia pastoris with a native extract [J].

Guillen, Marina ;

Dolors Benaiges, Maria ;

Valero, Francisco .

BIOCHEMICAL ENGINEERING JOURNAL, 2011, 54 (02) :117-123

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