Mycobacterium tuberculosis Rv1987 induces Th2 immune responses and enhances Mycobacterium smegmatis survival in mice

被引：17

作者：

Sha, Shanshan ^{[1
]}

Shi, Xiaoxia ^{[1
]}

Deng, Guoying ^{[2
]}

Chen, Lina ^{[3
]}

Xin, Yi ^{[4
]}

Ma, Yufang ^{[1
]}

机构：

[1] Dalian Med Univ, Dept Biochem & Mol Biol, 9 W Lushun South Rd, Dalian 116044, Peoples R China

[2] Dalian Med Univ, Dept Microbiol, Dalian 116044, Peoples R China

[3] Dalian Med Univ, Dept Pathol, Dalian 116044, Peoples R China

[4] Dalian Med Univ, Dept Biotechnol, Dalian 116044, Peoples R China

来源：

MICROBIOLOGICAL RESEARCH | 2017年 / 197卷

基金：

中国国家自然科学基金;

关键词：

Mycobacterium tuberculosis; Rv1987; Th2 immune response; Immune evasion; DENDRITIC CELLS; SECRETORY PROTEINS; T-CELLS; INTERLEUKIN-6; ANTIGENS; DIFFERENTIATION; IMMUNIZATION; NEUTROPHILS; MATURATION; INDUCTION;

D O I：

10.1016/j.micres.2017.01.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mycobacterium tuberculosis can interfere with host immune response and escape clearance through its specific antigens. M. tuberculosis Rv1987 encoded by region of difference (RD)-2 gene is a secretory protein with immunogenic potency. Here, we investigated the impact of Rv1987 on host cytokine responses and T cell polarization in mouse aerosol model. A recombinant M. smegmatis mc(2)155 strain that overexpressed Rv1987 protein (named MS1987) was constructed and used to infect C57BL/6 mice. The mc(2)155 harbored the empty vector (named MSVec) was as a control. The results showed that MS1987 challenged mice promoted Th2-biased cytokine responses with lower secretion of IFN-gamma but higher production of IL-4 and Rv1987-specific IgG antibody compared to MSVec infected mice. Neutrophilic inflammation and high bacterial burden were observed in the lung tissues of MS1987 infected mice probably own to the failed Thl cell immunity. Besides, subcutaneous injection of Rv1987 protein could mediate the Thi cytokine responses caused by M. bovis BCG in mice. These results indicated that M. tuberculosis Rv1987 protein could modulate host immune response towards Th2 profile, which probably contributed to the immune evasion of bacteria from host elimination. (C) 2017 Elsevier GmbH. All rights reserved.

引用

页码：74 / 80

页数：7

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