Selective removal of keratan sulfate in chondroitin sulfate samples by sequential precipitation with ethanol

被引：13

作者：

Galeotti, Fabio ^{[1
]}

Maccari, Francesca ^{[1
]}

Volpi, Nicola ^{[1
]}

机构：

[1] Univ Modena & Reggio Emilia, Dept Life Sci, I-41100 Modena, Italy

来源：

ANALYTICAL BIOCHEMISTRY | 2014年 / 448卷

关键词：

Chondroitin sulfate; Keratan sulfate; Ethanol precipitation; Hexosamines; Capillary electrophoresis; Glycosaminoglycans; DERMATAN SULFATE; OSTEOARTHRITIS; HEPARIN; QUALITY;

D O I：

10.1016/j.ab.2013.11.028

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Keratan sulfate (KS) is present as a contaminant in chondroitin sulfate (CS) mainly extracted from shark cartilage. We report a selective removal procedure of KS in CS samples by means of sequential precipitation with ethanol. Purified shark CS containing approximately 10% to 15% KS was subjected to a precipitation procedure in the presence of increasing percentages of saturated ethanol. In contrast to other solvents, 1.0 volume of ethanol was able to selectively purify CS, with a purity of approximately 100%, from KS. The current selective and simple procedure appears to be a reliable industrial preparation of CS devoid of large amounts of the residual KS. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：113 / 115

页数：3

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