Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay

被引：67

作者：

Lee, Jeong Hoon ^{[1
]}

Cosgrove, Benjamin D. ^{[2
]}

Lauffenburger, Douglas A. ^{[2
]}

Han, Jongyoon ^{[1
,2
]}

机构：

[1] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA

[2] MIT, Dept Biol Engn, Cambridge, MA 02139 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 30期

关键词：

PRECONCENTRATION; CELLS; SENSITIVITY; DYNAMICS; DEVICE;

D O I：

10.1021/ja902594f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this paper, we reported a simple, disposable PDMS micro/nanofluidic preconcentration chip for in vitro concentration-enhanced cell kinase assays. Utilizing the preconcentration (etectrokinetic trapping) directly from cell lysate (1 mM ATP) samples, we could achieve at least a 25-fold increase in reaction velocity and 65-fol.d enhancement in sensitivity. In addition, we shorten the assay time down to less than 10 min, with the sample volume requirements of down to similar to 5 cells. This device could be a generic and powerful tool for diagnostics and systems biology studies at the single-cell level, if property optimized and integrated with the cell culture microdevices.

引用

页码：10340 / +

页数：4

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