TU-tagging: cell type-specific RNA isolation from intact complex tissues

被引：1

作者：

Miller, Michael R. ^{[1
]}

Robinson, Kristin J. ^{[1
]}

Cleary, Michael D. ^{[1
]}

Doe, Chris Q. ^{[1
]}

机构：

[1] Univ Oregon, Howard Hughes Med Inst, Inst Mol Biol, Inst Neurosci, Eugene, OR 97403 USA

来源：

NATURE METHODS | 2009年 / 6卷 / 06期

基金：

美国国家卫生研究院;

关键词：

TRANSLATIONAL PROFILING APPROACH; URACIL PHOSPHORIBOSYLTRANSFERASE; GENE-EXPRESSION; DROSOPHILA; DECAY;

D O I：

10.1038/NMETH.1329

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We found that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil delivery can be used to label and purify cell type-specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates.

引用

页码：439 / U57

页数：5

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