Validation of a HPLC Method for the Determination of Metformin in Human Plasma and its Application to a Bioequivalence Study

被引：0
作者：
Kan, Xin [1 ,2 ]
Lin, Zhong [2 ]
Xiong, Jianhua [2 ]
Yang, Jinzhao [2 ]
Lin, Yuxian [2 ]
Li, Hao [2 ]
Chen, Dingwen [2 ]
Wang, Shujun [2 ]
机构：
[1] Yueqing Third Peoples Hosp, Yueqing 325604, Peoples R China
[2] Wenzhou Peoples Hosp, Wenzhou 325000, Peoples R China
来源：
LATIN AMERICAN JOURNAL OF PHARMACY | 2014年 / 33卷 / 05期
关键词：
Bioequivalence; HPLC; Metformin; Plasma; TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; PHARMACOKINETICS;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In this study, a simple, rapid and sensitive high performance liquid chromatography (HPLC) method is described for determination of metformin (MET) in plasma samples from bioequivalence assays. Sample preparation was accomplished through protein precipitation with 35% perchloric acid and dichloromethane, and chromatographic separation was performed on an Agilent ZORBAX SB-C18 column (150 x 4.6 mm, 5 mu m) at 35 degrees C. Mobile phase consisted of a mixture of acetonitrile-water-10 mM SDS-0.1% trifluoroacetic acid in water (35:47:13:5) at flow rate of 1.0 mLmin. Wavelength was set at 234 nm. The method was applied to a bioequivalence study of two drug products containing MET, and allowed determination of MET at low concentrations with a higher throughput than previously described methods.
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收藏
页码：835 / 840
页数：6
相关论文
共 11 条
[1]   Determination of metformin in plasma using a new ion pair solid phase extraction technique and ion pair liquid chromatography [J].
AbuRuz, S ;
Millership, J ;
McElnay, J .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2003, 798 (02) :203-209
[2]  
Arayne M Saeed, 2006, Pak J Pharm Sci, V19, P231
[3]  
El-Cindy A, 2010, J AOAC INT, V93, P1821
[4]   Fast liquid chromatographic-tandem mass spectrometric (LC-MS-MS) determination of metformin in plasma samples [J].
Heinig, K ;
Bucheli, F .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2004, 34 (05) :1005-1011
[5]   Pharmacokinetics and bioavailability of metformin in horses [J].
Hustace, Jaime L. ;
Firshman, Anna M. ;
Mata, John E. .
AMERICAN JOURNAL OF VETERINARY RESEARCH, 2009, 70 (05) :665-668
[6]   Simultaneous quantification of metformin and glipizide in human plasma by high-performance liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study [J].
Li, Ning ;
Deng, Ying ;
Qin, Feng ;
Yu, Jia ;
Li, Famei .
BIOMEDICAL CHROMATOGRAPHY, 2013, 27 (02) :191-196
[7]   HPLC-UV determination of metformin in human plasma for application in pharmacokinetics and bioequivalence studies [J].
Porta, Valentina ;
Schramm, Simone Grigoleto ;
Kano, Eunice Kazue ;
Koono, Eunice Emiko ;
Armando, Yara Popst ;
Fukuda, Kazuo ;
dos Reis Serra, Cristina Helena .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2008, 46 (01) :143-147
[8]   Prevention of Type 2 Diabetes and Its Complications in Developing Countries: A Review [J].
Rawal, Lal B. ;
Tapp, Robyn J. ;
Williams, Emily D. ;
Chan, Carina ;
Yasin, Shajahan ;
Oldenburg, Brian .
INTERNATIONAL JOURNAL OF BEHAVIORAL MEDICINE, 2012, 19 (02) :121-133
[9]   Simple and rapid method determination for metformin in human plasma using high performance liquid chromatography tandem mass spectrometry: Application to pharmacokinetic studies [J].
Sipoli Marques, Marlice A. ;
Soares, Alcenir de Souza ;
Pinto, Olivia Woyames ;
Werneck Barroso, Pedro Tupinamba ;
Pinto, Douglas Pereira ;
Ferreira-Filho, Milton ;
Werneck-Barroso, Eduardo .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 852 (1-2) :308-316
[10]   Rapid and simple high-performance liquid chromatographic assay for the determination of metformin in human plasma and breast milk [J].
Zhang, M ;
Moore, GA ;
Lever, M ;
Gardiner, SJ ;
Kirkpatrick, CMJ ;
Begg, EJ .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 766 (01) :175-179
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