The isolated perfused rat liver: standardization of a time-honoured model

被引:96
作者
Bessems, M.
t'Hart, N. A.
Tolba, R.
Doorschodt, B. M.
D Leuvenink, H. G.
Ploeg, R. J.
Minor, T.
van Gulik, T. M.
机构
[1] Univ Amsterdam, Acad Med Ctr, Dept Surg, Surg Lab,IWO 1151, NL-1105 AZ Amsterdam, Netherlands
[2] Univ Groningen Hosp, Surg Res Lab, Groningen, Netherlands
[3] Univ Bonn, Surg Clin, Div Surg Res, D-5300 Bonn, Germany
关键词
isolated perfused rat liver (IPRL); liver preservation; parameters; liver function; liver damage;
D O I
10.1258/002367706777611460
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
For many years, the isolated perfused rat liver (IPRL) model has been used to investigate the physiology and pathophysiology of the rat liver. This in vitro model provides the opportunity to assess cellular injury and liver function in an isolated setting. This review offers an update of recent developments regarding the IPRL set-up as well as the viability parameters that are used, with regards to liver preservation and ischaemia and reperfusion mechanisms. A review of the literature was performed into studies regarding liver preservation or liver ischaemia and reperfusion. An overview of the literature is given with particular emphasis on perfusate type and volume, reperfusion pressure, flow, temperature, duration of perfusion, oxygenation and on applicable viability parameters (liver damage and function). The choice of IPRL set-up depends on the question examined and on the parameters of interest. A standard technique is cannulation of the portal vein, bile duct and caval vein with pressure-controlled perfusion at 20 cm H2O (15 mmHg) to reach a perfusion flow of approximately 3 mL/min/g liver weight. The preferred perfusion solution is Krebs-Henseleit buffer, without albumin. The usual volume is 150-300 cm(3), oxygenated to a pO(2) of more than 500 mmHg. The temperature of the perfusate is maintained at 37 degrees C. Standardized markers should be used to allow comparison with other experiments.
引用
收藏
页码:236 / 246
页数:11
相关论文
共 69 条
[1]   Use of isolated perfused rat liver model for testing liver preservation solutions [J].
Ahmed, I ;
Attia, MS ;
Ahmad, N ;
Lodge, JPA ;
Potts, DJ .
TRANSPLANTATION PROCEEDINGS, 2001, 33 (7-8) :3709-3711
[2]   Hepatic function during prolonged isolated rat liver perfusion using a new miniaturized perfusion circuit [J].
Alexander, B ;
Aslam, M ;
Benjamin, IS .
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS, 1995, 34 (04) :203-210
[3]   Combined effects of fasting and alanine on liver function recovery after cold ischemia [J].
Arnault, I ;
Bao, YM ;
Dimicoli, JL ;
Lemoine, A ;
Sebagh, M ;
Adam, R .
TRANSPLANT INTERNATIONAL, 2002, 15 (2-3) :89-95
[4]   The effects of vascular bed expansion in steatotic rat liver graft viability [J].
Astarcioglu, H ;
Karademir, S ;
Atila, K ;
Sagol, Ö ;
Koçdor, H ;
Çoker, A ;
Astarcioglu, I .
TRANSPLANT INTERNATIONAL, 2004, 17 (04) :188-194
[5]   ENERGY CHARGE OF ADENYLATE POOL AS A REGULATORY PARAMETER . INTERACTION WITH FEEDBACK MODIFIERS [J].
ATKINSON, DE .
BIOCHEMISTRY, 1968, 7 (11) :4030-&
[6]   ALTERED THIOL AND CALCIUM HOMEOSTASIS IN OXIDATIVE HEPATOCELLULAR INJURY [J].
BELLOMO, G ;
ORRENIUS, S .
HEPATOLOGY, 1985, 5 (05) :876-882
[7]   PRINCIPLES OF SOLID-ORGAN PRESERVATION BY COLD-STORAGE [J].
BELZER, FO ;
SOUTHARD, JH .
TRANSPLANTATION, 1988, 45 (04) :673-676
[8]   EVALUATION OF PURINE NUCLEOSIDE PHOSPHORYLASE RELEASE AS A MEASURE OF HEPATIC ENDOTHELIAL-CELL INJURY [J].
BRASS, CA ;
MODY, MG .
HEPATOLOGY, 1995, 21 (01) :174-179
[9]   DIRECT AND INDIRECT MEASUREMENT OF THE HEPATIC EXTRACTION RATIO OF INDOCYANINE GREEN IN THE RAT [J].
BURNS, E ;
BALL, CE ;
CHRISTIE, JP ;
BROADHEAD, GD ;
TUCKER, GT ;
BAX, NDS .
CLINICAL SCIENCE, 1989, 76 (05) :503-508
[10]   REPERFUSION INJURY TO ENDOTHELIAL-CELLS FOLLOWING COLD ISCHEMIC STORAGE OF RAT LIVERS [J].
CALDWELLKENKEL, JC ;
CURRIN, RT ;
TANAKA, Y ;
THURMAN, RG ;
LEMASTERS, JJ .
HEPATOLOGY, 1989, 10 (03) :292-299