A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry

被引：8
作者：
Zhao, Wenbo [1 ]
Qin, Zhen [1 ]
Zhang, Chengsen [1 ]
Zhao, Meiping [1 ]
Luo, Hai [1 ]
机构：
[1] Peking Univ, Coll Chem & Mol Engn, MOE Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 69期
关键词：
EXONUCLEASE-III; SIGNAL AMPLIFICATION; RECYCLING AMPLIFICATION; BIOSENSOR; APTAMER; ASSAY; PROTEOMICS; STRATEGY;
D O I：
10.1039/c4cc03559c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A label-free double amplification system has been developed by using a ternary DNA probe containing the poly(adenine-thymine) sequence assisted by exonuclease III degradation. The method achieved more than 600-fold signal amplification and allowed sensitive detection of single-stranded DNA and thrombin at the pM level by using liquid chromatography/mass spectrometry.
引用
收藏
页码：9846 / 9848
页数：3
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