Tryptophan 13C nuclear-spin polarization generated by intraprotein electron transfer in a LOV2 domain of the blue-light receptor phototropin

被引：17

作者：

Eisenreich, Wolfgang ^{[2
]}

Fischer, Markus ^{[3
]}

Roemisch-Margl, Werner ^{[2
]}

Joshi, Monika ^{[2
]}

Richter, Gerald ^{[4
]}

Bacher, Adelbert ^{[2
]}

Weber, Stefan ^{[1
]}

机构：

[1] Univ Freiburg, Inst Phys Chem, D-79104 Freiburg, Germany

[2] Tech Univ Munich, Dept Chem, D-85748 Garching, Germany

[3] Univ Hamburg, Inst Lebensmittelchem, D-20146 Hamburg, Germany

[4] Cardiff Univ, Sch Chem, Cardiff CF10 3AT, S Glam, Wales

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2009年 / 37卷

关键词：

chemically induced dynamic nuclear polarization (CIDNP); electron transfer; flavin radical; nuclear-spin polarization; radical-pair mechanism; tryptophan radical; DNA PHOTOLYASE; C450A MUTANT; CRYPTOCHROMES; PLANTS; CIDNP;

D O I：

10.1042/BST0370382

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

C-13-NMR experiments were performed on photo-excited fully and partially C-13-labelled LOV2 domains of the blue-light receptor phototropin. In the present paper, we report on nuclear-spin polarized tryptophan resonances that are generated by light-induced intraprotein electron transfer to the FMN cofactor. The spectra are discussed with respect to earlier data obtained from C-13-NMR experiments on unlabelled LOV2 domains that have been reconstituted with FMN C-13 isotopologues.

引用

页码：382 / 386

页数：5

共 10 条

[1] Photochemically induced dynamic nuclear polarization in a C450A mutant of the LOV2 domain of the Avena sativa blue-light receptor phototropin
Richter, Gerald
Weber, Stefan
Römisch, Werner
Bacher, Adelbert
Fischer, Markus
Eisenreich, Wolfgang
Journal of the American Chemical Society, 2005, 127 (49): : 17245 - 17252
[2] Photochemically induced dynamic nuclear polarization in a C450A mutant of the LOV2 domain of the Avena sativa blue-light receptor phototropin
Richter, G
Weber, S
Römisch, W
Bacher, A
Fischer, M
Eisenreich, W
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (49) : 17245 - 17252
[3] Primary reactions of the LOV2 domain of phototropin, a plant blue-light photoreceptor
Kennis, JTM
Crosson, S
Gauden, M
van Stokkum, IHM
Moffat, K
van Grondelle, R
BIOCHEMISTRY, 2003, 42 (12) : 3385 - 3392
[4] Photochemically induced dynamic nuclear polarization in a C450A mutant of the LOV2 domain of the Avena sativa blue-light receptor phototropin (vol 127, pg 17245, 2005)
Richter, Gerald
Weber, Stefan
Romisch, Werner
Bacher, Adelbert
Fischer, Markus
Eisenreich, Wolfgang
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (22) : 7166 - 7166
[5] Vibration spectroscopy reveals light-induced chromophore and protein structural changes in the LOV2 domain of the plant blue-light receptor phototropin 1
Swartz, TE
Wenzel, PJ
Corchnoy, SB
Briggs, WR
Bogomolni, RA
BIOCHEMISTRY, 2002, 41 (23) : 7183 - 7189
[6] Coiled-coil dimerization of the LOV2 domain of the blue-light photoreceptor phototropin 1 from Arabidopsis thaliana
Halavaty, Andrei S.
Moffat, Keith
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2013, 69 : 1316 - 1321
[7] Detecting a New Source for Photochemically Induced Dynamic Nuclear Polarization in the LOV2 Domain of Phototropin by Magnetic-Field Dependent 13C NMR Spectroscopy
Kothe, Gerd
Lukaschek, Michail
Link, Gerhard
Kacprzak, Sylwia
Illarionov, Boris
Fischer, Markus
Eisenreich, Wolfgang
Bacher, Adelbert
Weber, Stefan
JOURNAL OF PHYSICAL CHEMISTRY B, 2014, 118 (40): : 11622 - 11632
[8] Primary photophysics of the FMN binding LOV2 domain of the plant blue light receptor phototropin of Avena sativa
Schüttrigkeit, TA
Kompa, CK
Salomon, M
Rüdiger, W
Michel-Beyerle, ME
CHEMICAL PHYSICS, 2003, 294 (03) : 501 - 508
[9] Photosensitivity of Kinase Activation by Blue Light Involves the Lifetime of a Cysteinyl-Flavin Adduct Intermediate, S390, in the Photoreaction Cycle of the LOV2 Domain in Phototropin, a Plant Blue Light Receptor
Okajima, Koji
Kashojiya, Sachiko
Tokutomi, Satoru
JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (49) : 40972 - 40981
[10] N- and C-terminal flanking regions modulate light-induced signal transduction in the LOV2 domain of the blue light sensor phototropin 1 from Avena sativa
Halavaty, Andrei S.
Moffat, Keith
BIOCHEMISTRY, 2007, 46 (49) : 14001 - 14009

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