Hepatitis E virus ORF1 encoded non structural protein-host protein interaction network

被引：13

作者：

Ojha, Nishant Kumar ^{[1
]}

Lole, Kavita S. ^{[1
]}

机构：

[1] Natl Inst Virol, Hepatitis Div, Microbial Containment Complex,Sus Rd, Pune 411021, Maharashtra, India

来源：

VIRUS RESEARCH | 2016年 / 213卷

关键词：

Yeast two hybrid; Virus-host interaction; Gene Ontology; SCALE MAP; REPLICATION; GENE; INFECTION; PATHWAY; CLASSIFICATION; LOCALIZATION; EXPRESSION; PRODUCT; ENZYME;

D O I：

10.1016/j.virusres.2015.12.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis E virus ORF1 encoded non-structural polyprotein (nsP) consist of multiple domains, namely: Methyltransferase, Y-domain, Protease, X-domain, Helicase and RNA dependent RNA polymerase. We have attempted to identify human liver cell proteins that are interacting with HEV ORF1 encoded functional domains by using Y2H screening. A total of 155 protein-protein interactions between HEV-ORF1 and human proteins were identified. Comparative analysis of the HEV-ORF1-Human interaction network with reconstructed human interactome showed that the cellular proteins interacting with HEV-ORF1 are central and interconnected. Enrichment analysis of Gene Ontology and cellular pathways showed that the viral proteins preferentially interacted with the proteins of metabolism and energy generation along with host immune response and ubiquitin proteasomal pathways. The mTOR and focal adhesion pathways were also targeted by the virus. These interactions suggest that HEV probably utilizes important proteins in carbohydrate metabolism, energy generation and iron homoeostasis in the host cells during its establishment. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：195 / 204

页数：10

共 61 条

[1] The 3′ end of hepatitis E virus (HEV) genome binds specifically to the viral RNA-dependent RNA polymerase (RdRp) [J].

Agrawal, S ;

Gupta, D ;

Panda, SK .

VIROLOGY, 2001, 282 (01) :87-101

[2]

[Anonymous], PLOS ONE

[3] Computing topological parameters of biological networks [J].

Assenov, Yassen ;

Ramirez, Fidel ;

Schelhorn, Sven-Eric ;

Lengauer, Thomas ;

Albrecht, Mario .

BIOINFORMATICS, 2008, 24 (02) :282-284

[4] Controlling the false discovery rate in behavior genetics research [J].

Benjamini, Y ;

Drai, D ;

Elmer, G ;

Kafkafi, N ;

Golani, I .

BEHAVIOURAL BRAIN RESEARCH, 2001, 125 (1-2) :279-284

[5] Gene Ontology Consortium: going forward [J].

Blake, J. A. ;

Christie, K. R. ;

Dolan, M. E. ;

Drabkin, H. J. ;

Hill, D. P. ;

Ni, L. ;

Sitnikov, D. ;

Burgess, S. ;

Buza, T. ;

Gresham, C. ;

McCarthy, F. ;

Pillai, L. ;

Wang, H. ;

Carbon, S. ;

Dietze, H. ;

Lewis, S. E. ;

Mungall, C. J. ;

Munoz-Torres, M. C. ;

Feuermann, M. ;

Gaudet, P. ;

Basu, S. ;

Chisholm, R. L. ;

Dodson, R. J. ;

Fey, P. ;

Mi, H. ;

Thomas, P. D. ;

Muruganujan, A. ;

Poudel, S. ;

Hu, J. C. ;

Aleksander, S. A. ;

McIntosh, B. K. ;

Renfro, D. P. ;

Siegele, D. A. ;

Attrill, H. ;

Brown, N. H. ;

Tweedie, S. ;

Lomax, J. ;

Osumi-Sutherland, D. ;

Parkinson, H. ;

Roncaglia, P. ;

Lovering, R. C. ;

Talmud, P. J. ;

Humphries, S. E. ;

Denny, P. ;

Campbell, N. H. ;

Foulger, R. E. ;

Chibucos, M. C. ;

Giglio, M. Gwinn ;

Chang, H. Y. ;

Finn, R. .

NUCLEIC ACIDS RESEARCH, 2015, 43 (D1) :D1049-D1056

[6] High mortality associated with an outbreak of hepatitis E among displaced persons in Darfur, Sudan [J].

Boccia, Delia ;

Guthmann, Jean-Paul ;

Klovstad, Hilde ;

Hamid, Nuha ;

Tatay, Mercedes ;

Ciglenecki, Iza ;

Nizou, Jacques-Yves ;

Nicand, Elisabeth ;

Guerin, Philippe Jean .

CLINICAL INFECTIOUS DISEASES, 2006, 42 (12) :1679-1684

[7] Mapping of Chikungunya Virus Interactions with Host Proteins Identified nsP2 as a Highly Connected Viral Component [J].

Bourai, Mehdi ;

Lucas-Hourani, Marianne ;

Gad, Hans Henrik ;

Drosten, Christian ;

Jacob, Yves ;

Tafforeau, Lionel ;

Cassonnet, Patricia ;

Jones, Louis M. ;

Judith, Delphine ;

Couderc, Therese ;

Lecuit, Marc ;

Andre, Patrice ;

Kuemmerer, Beate Mareike ;

Lotteau, Vincent ;

Despres, Philippe ;

Tangy, Frederic ;

Vidalain, Pierre-Olivier .

JOURNAL OF VIROLOGY, 2012, 86 (06) :3121-3134

[8] Molecular biology and replication of hepatitis E virus [J].

Cao, Dianjun ;

Meng, Xiang-Jin .

EMERGING MICROBES & INFECTIONS, 2012, 1

[9] mTOR: taking cues from the immune microenvironment [J].

Delgoffe, Greg M. ;

Powell, Jonathan D. .

IMMUNOLOGY, 2009, 127 (04) :459-465

[10] Analysis of Antiviral Response in Human Epithelial Cells Infected with Hepatitis E Virus [J].

Devhare, Pradip B. ;

Chatterjee, Subhashis N. ;

Arankalle, Vidya A. ;

Lole, Kavita S. .

PLOS ONE, 2013, 8 (05)

← 1 2 3 4 5 6 7 →