Encapsulation of Bacterial Cells in Electrospun Microtubes

被引:81
作者
Klein, S. [2 ]
Kuhn, J. [3 ]
Avrahami, R. [1 ]
Tarre, S. [2 ]
Beliavski, M. [2 ]
Green, M. [2 ]
Zussman, E. [1 ]
机构
[1] Technion Israel Inst Technol, Fac Mech Engn, IL-32000 Haifa, Israel
[2] Technion Israel Inst Technol, Fac Civil & Environm Engn, IL-32000 Haifa, Israel
[3] Technion Israel Inst Technol, Fac Biol, IL-32000 Haifa, Israel
关键词
LIVING ORGANISMS; NANOFIBERS; MICROORGANISMS;
D O I
10.1021/bm900168v
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Encapsulation of whole microbial cells in microtubes for use in bioremediation of Pollutants in water systems was the main focus of this investigation. Coelectrospinning of a core polymeric solution with bacterial cells and a shell polymer solution using a spinneret with two coaxial capillaries resulted in microtubes with porous walls. The ability of the microtube's structure to support cell attachment and maintain enzymatic activity and proliferation of the encapsulated microbial cells was examined. The results obtained show that the encapsulated cells maintain some of their phosphatase, beta-galactosidase and denirification activity and are able to respond to conditions that induce these activities. This study demonstrates electrospun microtubes are a suitable platform for the immobilization of intact microbial cells.
引用
收藏
页码:1751 / 1756
页数:6
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