A laccase from the medicinal mushroom Ganoderma lucidum

被引：59

作者：

Wang, X.

Ng, T. B. ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Fac Med, Dept Biochem, Shatin, Hong Kong, Peoples R China

[2] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

[3] China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R China

[4] Minist Agr, Beijing, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2006年 / 72卷 / 03期

关键词：

D O I：

10.1007/s00253-006-0314-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A protein demonstrating laccase activity and potent inhibitory activity towards human immunodeficiency virus (HIV)-1 reverse transcriptase (IC50 1.2 mu M) was isolated from fresh fruiting bodies of the medicinal mushroom Ganoderma lucidum. The laccase had a novel N-terminal sequence and a molecular mass of 75 kDa, which is higher than the range (55-56 kDa) reported for most other mushroom laccases. It was isolated by sequential chromatography on DEAE-cellulose and Affi-gel blue gel and adsorption on Con A-Sepharose. Unlike some of the previously isolated laccases, it was adsorbed only on Con A-Sepharose. The enzyme required a pH of 3-5 and a temperature of 70 degrees C to exhibit maximal activity. Minimal activity was detected at pH 6 and 7. Activity was undetectable at pH 8 and 9 and after exposure to 100 degrees C for 10 min.

引用

页码：508 / 513

页数：6

共 48 条

[1] IMMOBILIZED LACCASE FOR PHENOLIC REMOVAL IN MUST AND WINE [J].