LC-MS/MS DETECTION AND ANALYSIS OF FURAN METABOLITE RESIDUES IN PROPOLIS

In this paper, the high-sensitivity and high-selective fluorescence-derived reagents were used for pre-column derivatization and high-performance liquid chromatography to detect the metabolites of nitrofuran antibiotics. The method of liquid chromatography-tandem mass spectrometry for the determination of nitrofuran metabolites in bee pollen was established. The sample was acidified by perchloric acid, purified by solid phase extraction, derivatized, extracted with ethyl acetate, and analyzed by tandem mass spectrometry. At the three levels of 1.0, 2.0, 5.0 mu g/kg, the average recovery of nitrofuran metabolites ranged from 93.7% to 97.2%, the intra-day relative standard deviation was less than 10%, and the relative standard deviation during the day was less than 13.5%. It has good linearity in the range of 0.5-20 ng/mL (r>0.99 detection limit is 0.25 mu g/kg, and the limit of quantification is 1.0 mu g/kg. The detection method is simple, the selectivity is good, the anti-interference ability is strong, and it is suitable for bee pollen. Analysis of the nitrofuran metabolites was confirmed.