Detection of anti-erythropoietin antibodies in haemodialysis patients treated with recombinant human-erythropoietin

被引：39

作者：

Castelli, G

Famularo, A

Semino, C

Machi, AM

Ceci, A

Cannella, G

Melioli, G

机构：

[1] Ist Nazl Ricerca Cancro, Sez Aggregata Immunoterapia Cellulare, I-18132 Genoa, Italy

[2] Azienda Osped S Martino, Div Nefrol & Emodialisi, Genoa, Italy

[3] Azienda Osped S Martino, Lab Anal, Genoa, Italy

[4] Ist Nazl Ricerca Cancro, Serv Nuovi Farm, I-18132 Genoa, Italy

来源：

PHARMACOLOGICAL RESEARCH | 2000年 / 41卷 / 03期

关键词：

erythropoietin; immunoglobulins; enzyme-immunoassay;

D O I：

10.1006/phrs.1999.0593

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

An enzyme-immunoassay was developed to evaluate the presence of anti-erythropoietin antibodies in:plasma samples obtained from renal failure patients treated with recombinant human erythropoietin (rh-EPO). The assay was specific and reproducible. Normal donors had no antibodies to EPO, while 67% of treated patients were positive to the assay. While the specificity of anti-EPO IgG antibodies was high, their affinity for the antigen was low. This finding can be explained by the very small differences in the structure of rh-EPO compared to that of natural EPO. The assay described could be useful in evaluating the long-term effects of rh-EPO treatment on the control of anaemia in renal failure patients. (C) 2000 Academic Press.

引用

收藏

页码：313 / 318

页数：6

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