Characterisation of hyaluronic acid and chondroitin/dermatan sulfate from the lumpsucker fish, C. lumpus

被引：22
作者：
Panagos, Charalampos G. [1 ]
Thomson, Derek [2 ]
Moss, Claire [2 ]
Bavington, Charles D. [2 ]
Olafsson, Halldor G. [3 ]
Uhrin, Dusan [1 ]
机构：
[1] EaStCHEM Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland
[2] GlycoMar Ltd, European Ctr Marine Biotechnol, Oban PA37 1QA, Argyll, Scotland
[3] Biopol, IS-545 Skagastrond, Iceland
来源：
CARBOHYDRATE POLYMERS | 2014年 / 106卷
基金：
英国工程与自然科学研究理事会;
关键词：
Cyclopterus lumpus; Chondroitin sulfate; Dermatan sulfate; Hyaluronic acid; Glycosaminoglycan; NMR; MASS-SPECTROMETRIC ANALYSIS; DERMATAN SULFATE; SEA-CUCUMBER; STRUCTURAL-CHARACTERIZATION; ANTIINFLAMMATORY ACTIVITY; ANTICOAGULANT ACTIVITIES; MARINE-INVERTEBRATES; PROTEIN INTERACTIONS; CONNECTIVE-TISSUE; HEPARAN-SULFATE;
D O I：
10.1016/j.carbpol.2014.01.090
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The lumpsucker, Cyclopterus lumpus, a cottoid teleost fish found in the cold waters of the North Atlantic, and North Pacific, was identified as a possible source of GAGs. The GAGs present in the C. lumpus dorsal hump and body wall tissue were isolated and purified. Two fractions were analysed by NMR and their GAG structures determined as hyaluronic acid and CS/DS chains. The latter fraction contained GIcA (65% of the total uronic acids) and IdoA (the remaining 35%). All uronic acid residues were unsulfated, whilst 86% of the GalNAc was 4-sulfated and 14% was 6-sulfated. The presence of GlcA-GalNAc4S, IdoA-GalNAc4S and GlcA-GalNAc6S disaccharide fragments was confirmed. The isolated GAGs obtained from each tissue were biochemically characterised. The lumpsucker offers a high yield source of GAGs, which compares favourably with other sources such as shark cartilage. (c) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：25 / 33
页数：9
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