DEVELOPMENT AND OPTIMIZATION OF A STANDARD METHOD FOR EXTRACTION OF MICROPLASTICS IN MUSSELS BY ENZYME DIGESTION OF SOFT TISSUES

被引：240
作者：
Catarino, Ana I. [1 ]
Thompson, Richard [2 ]
Sanderson, William [1 ,3 ]
Henry, Theodore B. [1 ,4 ,5 ]
机构：
[1] Heriot Watt Univ, Inst Life & Earth Sci, Sch Energy Geosci Infrastruct & Soc, Edinburgh, Midlothian, Scotland
[2] Univ Plymouth, Sch Marine Sci & Engn, Plymouth, Devon, England
[3] St Abbs Marine Stn, St Abbs, Berwick, England
[4] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN USA
[5] Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37932 USA
来源：
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY | 2017年 / 36卷 / 04期
关键词：
Enzymatic digestion; Fourier transform infrared (FT-IR); Microplastics; Mussels; Emerging pollutants; Environmental toxicology; MARINE; SEDIMENTS; PROTOCOL; FIBERS; FISH;
D O I：
10.1002/etc.3608
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 +/- 10%) for NaOH and enzyme digestions. The authors recommend use of industrial enzymes based on digestion efficiency, microplastic recovery, and avoidance of caustic chemicals. (C) 2016 SETAC
引用
收藏
页码：947 / 951
页数：5
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