Confocal microscopy of cytoplasmic lipid droplets in a live cancer cell: number, polarity, diffusion and solvation dynamics

被引：36

作者：

Chowdhury, Rajdeep ^{[1
]}

Jana, Batakrishna ^{[2
]}

Saha, Abhijit ^{[2
]}

Ghosh, Surajit ^{[2
]}

Bhattacharyya, Kankan ^{[1
]}

机构：

[1] Indian Assoc Cultivat Sci, Dept Chem Phys, Kolkata 700032, India

[2] CSIR, Indian Inst Chem Biol, Div Chem, Kolkata 700032, India

来源：

MEDCHEMCOMM | 2014年 / 5卷 / 04期

关键词：

NILE RED; COUMARIN-153; WATER;

D O I：

10.1039/c3md00269a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Time resolved confocal microscopy indicates that the cytoplasmic lipid droplets (CLDs) in live cells (normal and cancer lung cells) are less polar, and exhibit slower diffusion (motility) and solvation dynamics than the cytoplasm. The number of CLDs in a human lung cancer cell (A549) is similar to 10 times higher than in a non-cancer lung fibroblast cell (WI38). This may result in accumulation of non-polar cell signaling agents in the CLDs of the cancer cell.

引用

页码：536 / 539

页数：4

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