Oncogenic long noncoding RNA MALAT1 and HCV-related hepatocellular carcinoma

被引:49
作者
Toraih, Eman A. [1 ,2 ]
Ellawindy, Alia [1 ]
Fala, Salma Y. [3 ]
Al Ageeli, Essam [4 ]
Gouda, Nawal S. [5 ]
Fawzy, Manal S. [6 ,7 ]
Hosny, Somaya [2 ,8 ]
机构
[1] Suez Canal Univ, Fac Med, Dept Histol & Cell Biol, Genet Unit, Ismailia, Egypt
[2] Suez Canal Univ, Ctr Excellence Mol & Cellular Med, Ismailia, Egypt
[3] Suez Canal Univ, Fac Med, Ismailia, Egypt
[4] Jazan Univ, Fac Med, Dept Clin Biochem Med Genet, PO 45142, Jazan, Saudi Arabia
[5] Mansoura Univ, Fac Med, Dept Med Microbiol & Immunol, Mansoura, Egypt
[6] Suez Canal Univ, Fac Med, Dept Med Biochem, PO 41522, Ismailia, Egypt
[7] Northern Border Univ, Dept Biochem, Fac Med, Ar Ar, Saudi Arabia
[8] Suez Canal Univ, Dept Histol & Cell Biol, Fac Med, Ismailia, Egypt
关键词
MALAT1; HCV; HCC; lncRNA; Meta-analysis; ADENOCARCINOMA TRANSCRIPT 1; COMPETING ENDOGENOUS RNA; LNCRNA MALAT1; CANCER PROGRESSION; CELL-PROLIFERATION; DOWN-REGULATION; UP-REGULATION; LUNG-CANCER; PROMOTES; METASTASIS;
D O I
10.1016/j.biopha.2018.03.105
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related mortality worldwide. The oncogenic function of the long non-coding RNA; metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in HCC remains unclear. We aimed to evaluate MALAT1 serum expression profile in HCC and explore its relation to the clinicopathological features. Quantitative Real Time-Polymerase Chain Reaction was applied in 70 cohorts (30 HCC, 20 HCV, 20 controls). Further meta-analysis of clinical studies and in vitro validated experiments was employed. Serum MALAT1 showed area under the curve of 0.79 and 0.70 to distinguish patients with cancer from normal and cirrhotic individuals at fold change of 1.0 and 1.26, respectively. Expression level was significantly higher in males (P < 0.001) and patients with massive ascites (P = 0.005). Correlation analysis showed positive correlation of MALAT1 with total bilirubin (r = 0.456, P < 0.001) and AST (r = 0.280, P = 0.019), and negative correlation with the hemoglobin level (r = 0.312, P = 0.009). Meta-analysis showed that the over-expressed MALAT1 was linked to tumor number [Cohen's d = 0.450, 95% CI (0.21 to 0.68)], clinical stage [Cohen's d = 0.048, 95% CI (-0.83 to 0.74)], and AFP level [Cohen's d = 0.354, 95% CI (0.1 to 0.57)]. In silico data analysis and systematic review confirmed MALAT1 oncogenic function in cancer development and progression. In conclusion, circulatory MALAT1 might represent a putative non-invasive prognostic biomarker indicating worse liver failure score in HCV-related HCC patients with traditional markers. Large-scale verification is warranted in future studies.
引用
收藏
页码:653 / 669
页数:17
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