Validated simultaneous HPTLC analysis of scopoletin and gallic acid in the methanolic fraction of Jatropha glandulifera

A high-performance thin-layer chromatography method for the simultaneous quantitative determination and validation of scopoletin and gallic acid in the methanolic fraction of Jatropha glandulifera L. was developed for the first time. For achieving good separation, the mobile phase of toluene-ethyl acetate-glacial acetic acid (7.5:2.5:0.1, V/V) was used. Densitometric determination was carried out at 254 nm for scopoletin and gallic acid, in reflection/absorption mode; the calibration curves were linear in the range of 100-600 ng per spot. During the analysis, the methanolic fraction of J. glandulifera L. showed the presence of scopoletin (0.08%) and gallic acid (0.04%). The proposed method is simple, precise, specific, and accurate. The obtained data can be used for the routine analysis of reported biomarkers in crude drug and extracts. The simultaneous quantification and method validation of these biomarkers have not yet been reported in J. glandulifera L. which may be utilized for the proper standardization of the plant.