Prevalence Rates of Escherichia coli O157:H7 and Salmonella at Different Sampling Sites on Cattle Hides at a Feedlot and Processing Plant

被引:23
作者
Kalchayanand, Norasak [1 ]
Brichta-Harhay, Dayna M. [1 ]
Arthur, Terrance M. [1 ]
Bosilevac, Joseph M. [1 ]
Guerini, Michael N. [1 ]
Wheeler, Tommy L. [1 ]
Shackelford, Steven D. [1 ]
Koohmaraie, Mohammad [1 ]
机构
[1] USDA ARS, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA
关键词
AEROBIC-BACTERIA; IMMUNOMAGNETIC SEPARATION; ORAL-CAVITY; BEEF-CATTLE; O157-H7; CARCASSES; ENTEROBACTERIACEAE; FECES; SURFACES;
D O I
10.4315/0362-028X-72.6.1267
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The prevalence rates of Escherichia coli O157:H7 and Salmonella at different sampling sites on cattle hides were determined at a feedlot and a processing plant. Sponge samples were collected from six hide surface sites at the feedlot (left and right shoulders, left and right ribs, back, and belly) and four sites at the processing plant (left and right shoulders, back, and belly). The prevalence of E. coli O157:H7 was approximately 80% for left and right shoulder and rib samples, 68% for back samples, and 92% for belly samples collected at the feedlot. At the processing plant, the prevalences of E. coli O157:H7 at all four sites were between 76 and 79%. Salmonella prevalence in feedlot samples was too low to allow for accurate analysis. The prevalence of Salmonella at processing was 49% for left shoulder samples, 49% for right shoulder samples, 40% for back samples, and 68% for belly samples. The results of this study indicate that the site most likely to be naturally contaminated with E. coli O157:H7 and Salmonella simultaneously was the belly.
引用
收藏
页码:1267 / 1271
页数:5
相关论文
共 19 条
[1]   Transportation and lairage environment effects on prevalence, numbers, and diversity of Escherichia coli O157:H7 on hides and carcasses of beef cattle at processing [J].
Arthur, Terrance M. ;
Bosilevac, Joseph M. ;
Brichta-Harhay, Dayna M. ;
Guerini, Michael N. ;
Kalchayanand, Norasak ;
Shackelford, Steven D. ;
Wheeler, Tommy L. ;
Koohmaraie, Mohammad .
JOURNAL OF FOOD PROTECTION, 2007, 70 (02) :280-286
[2]   Escherichia coli O157 prevalence and enumeration of aerobic bacteria, Enterobacteriaceae, and Escherichia coli O157 at various steps in commercial beef processing plants [J].
Arthur, TM ;
Bosilevac, JM ;
Nou, XW ;
Shackelford, SD ;
Wheeler, TL ;
Kent, MP ;
Jaroni, D ;
Pauling, B ;
Allen, DM ;
Koohmaraie, M .
JOURNAL OF FOOD PROTECTION, 2004, 67 (04) :658-665
[3]   Methods for recovering Escherichia coli O157:H7 from cattle fecal, hide, and carcass samples:: Sensitivity and improvements [J].
Barkocy-Gallagher, GA ;
Edwards, KK ;
Nou, X ;
Bosilevac, JM ;
Arthur, TM ;
Shackelford, SD ;
Koohmaraie, M .
JOURNAL OF FOOD PROTECTION, 2005, 68 (11) :2264-2268
[4]   Seasonal prevalence of Shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants [J].
Barkocy-Gallagher, GA ;
Arthur, TM ;
Rivera-Betancourt, M ;
Nou, XW ;
Shackelford, SD ;
Wheeler, TL ;
Koohmaraie, M .
JOURNAL OF FOOD PROTECTION, 2003, 66 (11) :1978-1986
[5]   Prevalence of Escherichia coli O157 and levels of aerobic bacteria and Enterobacteriaceae are reduced when hides are washed and treated with cetylpyridinium chloride at a commercial beef processing plant [J].
Bosilevac, JM ;
Arthur, TM ;
Wheeler, TL ;
Shackelford, SD ;
Rossman, M ;
Reagan, JO ;
Koohmaraie, M .
JOURNAL OF FOOD PROTECTION, 2004, 67 (04) :646-650
[6]   Salmonella and Escherichia coli O157:H7 contamination on hides and carcasses of cull cattle presented for slaughter in the United States:: an evaluation of prevalence and bacterial loads by Immunomagnetic separation and direct plating methods [J].
Brichta-Harhay, Dayna M. ;
Guerini, Michael N. ;
Arthur, Terrance M. ;
Bosilevac, Joseph M. ;
Kalchayanand, Norasak ;
Shackelford, Steven D. ;
Wheeler, Tommy L. ;
Koohmaraie, Mohammad .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (20) :6289-6297
[7]   Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing [J].
Elder, RO ;
Keen, JE ;
Siragusa, GR ;
Barkocy-Gallagher, GA ;
Koohmaraie, M ;
Laegreid, WW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (07) :2999-3003
[8]  
Fliess J.L., 1981, STAT METHODS RATES P, V2nd
[9]  
Grandin T., 1990, proceedings of the Livestock Conservation Institute, P115
[10]   Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by a multiplex PCR [J].
Hu, Y ;
Zhang, Q ;
Meitzler, JC .
JOURNAL OF APPLIED MICROBIOLOGY, 1999, 87 (06) :867-876