Scotin, a novel p53-inducible proapoptotic protein located in the ER and the nuclear membrane

被引：97

作者：

Bourdon, JC ^{[1
]}

Renzing, J ^{[1
]}

Robertson, PL ^{[1
]}

Fernandes, KN ^{[1
]}

Lane, DP ^{[1
]}

机构：

[1] Univ Dundee, Dept Surg & Mol Oncol, Ninewells Hosp & Med Sch, CRC Cell Transformat Res Grp, Dundee DD1 9SY, Scotland

来源：

JOURNAL OF CELL BIOLOGY | 2002年 / 158卷 / 02期

关键词：

transactivation; p53-binding site; cell death; cancer; 3p21;

D O I：

10.1083/jcb.200203006

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

p53 is a transcription factor that induces growth arrest or apoptosis in response to cellular stress. To identify new p53-inducible proapoptotic genes, we compared, by differential display, the expression of genes in spleen or thymus of normal and p53 nullizygote mice after gamma-irradiation of whole animals. We report the identification and characterization of human and mouse Scotin homologues, a novel gene directly transactivated by p53. The Scotin protein is localized to the ER and the nuclear membrane. Scotin can induce apoptosis in a caspase-dependent manner. Inhibition of endogenous Scotin expression increases resistance to p53-dependent apoptosis induced by DNA damage, suggesting that Scotin plays a role in p53-dependent apoptosis. The discovery of Scotin brings to light a role of the ER in p53-dependent apoptosis.

引用

页码：235 / 246

页数：12

共 42 条

[1] Transcriptional activation by p53, but not induction of the p21 gene, is essential for oncogene-mediated apoptosis [J].