Multifunctional nanoplatform for dual-mode sensitive detection of pathogenic bacteria and the real-time bacteria inactivation

被引:54
|
作者
Hao, Zhe [1 ]
Lin, Xiaodong [1 ]
Li, Jinjie [1 ]
Yin, Yanliang [1 ]
Gao, Xia [1 ]
Wang, Shuo [3 ]
Liu, Yaqing [1 ,2 ]
机构
[1] Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, State Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China
[2] Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100037, Peoples R China
[3] Nankai Univ, Sch Med, Tianjin Key Lab Food Sci & Hlth, Tianjin 300071, Peoples R China
来源
BIOSENSORS & BIOELECTRONICS | 2021年 / 173卷
基金
中国国家自然科学基金;
关键词
Bacterial infection; Point-of-care testing (POCT); Dual-mode portable detection; Antibacteria; Photothermal therapy; PRUSSIAN BLUE NANOPARTICLES; LATERAL FLOW ASSAY; OF-CARE DETECTION; PHOTOTHERMAL ABLATION; GOLD NANOPARTICLES; READOUT; GENERATION; BENEFITS; AGENTS;
D O I
10.1016/j.bios.2020.112789
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Bacterial infection is a growing public health concern and causes a huge medical and financial burden. It is of significance to efficiently construct multifunctional platforms for bacterial point-of-care testing (POCT) and elimination. Herein, near-infrared (NIR) light-responded vancomycin-doped prussian blue nanoparticles (PB-VAN(NPs)) with high efficient photothermal conversion was synthesized for binding, dual-mode portable detection, and elimination of bacteria. The PB-VAN(NPs) can bind to the surface of Gram-positive bacteria such as Staphylococcus aureus (S. aureus), forming complex of PB-VAN(NPs)/S. aureus. After being centrifugated, the suspension solution of PB-VAN(NPs) can stimulate perfluorohexane (PFH) to rapidly release oxygen (O-2) under NIR irradiation. Thus, the bacteria can be sensitively detected with portable pressure meter as signal reader, reporting a limit of detection (LOD) of 1.0 CFU mL(-1). On the other side, the sediment of PB-VAN(NPs)/S. aureus can be detected via thermal camera, reporting a LOD of 1.0 CFU mL(-1). Interestingly, the bacteria can be effectively inactivated with the local temperature elevation during temperature-based detection. The antibacterial efficiency reaches as high as 99.8%. The developed multifunctional nanoplatform not only provides a straightforward "mix-then-test" way for portable detection of bacteria with high sensitivity, also realizes high efficiency elimination of bacteria simultaneously. The developed strategy was further applied for promoting wound healing of bacteria-infected mice.
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页数:9
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