New possibilities of matrix-assisted laser desorption ionization time of flight mass spectrometry to analyze barley malt quality.: Highly sensitive detection of mycotoxins

被引:10
作者
Blechova, Petra
Havlova, Pavia
Gajdosova, Dagmar
Havel, Josef
机构
[1] Masaryk Univ, Fac Sci, Dept Analyt Chem, Brno 61137, Czech Republic
[2] Malting Inst Brno, Res Inst Brewing & Malting, Brno 61400, Czech Republic
关键词
barley; malt; trichothecene mycotoxins; deoxynivalenol; nivalenol; MALDI-TOF mass spectrometry; matrices;
D O I
10.1002/tox.20195
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The occurrence of mycotoxins in agricultural commodities is a major health concern for livestock, humans, and the environment. Barley and subsequently malt quality is of fundamental importance to obtain good quality beer. Classical methods of analysis often require tedious, laborious, and expensive processes. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) instrumentation enables highly sensitive and fast analysis and/or detection using a very small sample. The possibilities of MALDI-TOF MS for he identification and/or detection of trichothecene mycotoxins (deoxynivalenol (DON) and nivalenol (NIV), respectively) in barley malt were examined, and it was found that almost all classical MALDI matrices failed to ionize the compounds being studied. This detailed study of the ionization conditions and the search for unconventional matrices led to the discovery of suitable MALDI matrices, which enable ionization of trichothecene mycotoxins. These were: fine powdered synthetic diamond, sodium azide, or hydrazine hydrate. It is possible to detect 8.5 x 10(-12) mol (2.5 ng) of deoxynivalenol or 64 x 10(-12) mol (20 ng) of nivalenol in just 1 mu L of barley malt extract (equivalent to 600 mu g of DON in 1 kg of barley malt). The procedure developed enables fast determination of DON and NIV in barley, malt, or similar products. (c) 2006 Wiley Periodicals, Inc.
引用
收藏
页码:403 / 408
页数:6
相关论文
共 31 条
[1]   Rapid simultaneous determination of major type A- and B-trichothecenes as well as zearalenone in maize by high performance liquid chromatography-tandem mass spectrometry [J].
Berthiller, F ;
Schuhmacher, R ;
Buttinger, G ;
Krska, R .
JOURNAL OF CHROMATOGRAPHY A, 2005, 1062 (02) :209-216
[2]   Determination of type B trichothecenes and macrocyclic lactone mycotoxins in field contaminated maize [J].
Cavaliere, C ;
D'Ascenzo, G ;
Foglia, P ;
Pastorini, E ;
Samperi, R ;
Laganà, A .
FOOD CHEMISTRY, 2005, 92 (03) :559-568
[3]  
FURLONG EB, 1995, J AOAC INT, V78, P386
[4]  
HASTINGS DJ, 1995, J AM SOC BREW CHEM, V53, P78
[5]  
Josephs RD, 1997, CEREAL RES COMMUN, V25, P363
[6]   Determination of trichothecene mycotoxins in wheat by use of supercritical fluid extraction and high-performance liquid chromatography with diode array detection or gas chromatography with electron capture detection [J].
Josephs, RD ;
Krska, R ;
Grasserbauer, M ;
Broekaert, JAC .
JOURNAL OF CHROMATOGRAPHY A, 1998, 795 (02) :297-304
[7]   State of the art of trichothecenes analysis [J].
Koch, P .
TOXICOLOGY LETTERS, 2004, 153 (01) :109-112
[8]   Determination of trichothecenes in cereals [J].
Kotal, F ;
Holadová, K ;
Hajslová, J ;
Poustka, J ;
Radová, Z .
JOURNAL OF CHROMATOGRAPHY A, 1999, 830 (01) :219-225
[9]   The state-of-the-art in the analysis of type-A and -B trichothecene mycotoxins in cereals [J].
Krska, R ;
Baumgartner, S ;
Josephs, R .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 2001, 371 (03) :285-299
[10]   Performance of modern sample preparation techniques in the analysis of Fusarium mycotoxins in cereals [J].
Krska, R .
JOURNAL OF CHROMATOGRAPHY A, 1998, 815 (01) :49-57