The concentrations of five different kinds of environmental hormone which including nonyl phenol, bis-phenol A, methyltestosterone, megestrol and diethylstilbestrol in aquaculture seawater of Shenzhen have been studied. Their risk quotients (RQ) are calculated to evaluate the risk to seawater pollution. The results showed that the RQs for the five environment hormones are nonyl phenol (0 - 0.07), bis-phenol (0 - 2.15), methyltestosterone (0.17), diethylstilbestrol and megestrol (not found). Bis-phenol belongs to chronic potential ecological risk in the water and sediment. The purpose is to provide clues for improving Chinese Eco-risk assessment techniques and thus to enhance security of mariculture in China.