Establishment of reference interval for immature platelet fraction using Sysmex XN9000 hematology analyzer in Anhui province in China

The aim of this study was to establish reference intervals for immature platelet fraction (IPF) for healthy adults and term newborns in the area of Anhui province. Except outliers, a total of 2179 health adult examination subjects and 153 healthy term newborns were included in this study. The percentage (IPF%) and absolute value (IPF#) of IPF in adult peripheral blood and neonatal umbilical cord blood were measured using the Sysmex XN9000 fully automatic hematology analyzer. The reference intervals were calculated by non-parametric percentile method according to the Clinical and Laboratory Standard Institute guideline C28-A3. Our results showed that, for adults, no significant differences were found between males and females, while significant difference existed between different age groups for IPF% and IPF#. A significant difference also existed between adults and term newborns for all test parameters. The reference intervals of IPF% and IPF# for adults and term newborns were 0.7-8.4% and 2.9-16.9x109/L, 0.9-4.8% and 3.1-13.5x109/L, respectively. The reference intervals determined by the Sysmex XN9000 hematology analyzer for healthy adults and term newborns in the area of Anhui province would be useful for future clinical diagnosis as well as treatment.