Myricetin enhances osteogenic differentiation through the activation of canonical Wnt/β-catenin signaling in human bone marrow stromal cells
被引:26
|
作者:
Ying, Xiaozhou
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Peoples R ChinaWenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Ying, Xiaozhou
[1
,2
]
Chen, Xiaowei
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Med Univ, Affiliated Hosp 1, Dept Ultrasound, Wenzhou 325000, Peoples R ChinaWenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Chen, Xiaowei
[3
]
Feng, Yongzeng
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Peoples R ChinaWenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Feng, Yongzeng
[1
,2
]
Xu, Huazi
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Peoples R ChinaWenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Xu, Huazi
[1
,2
]
Chen, Hua
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Peoples R ChinaWenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Chen, Hua
[1
,2
]
Yu, Kehe
论文数: 0引用数: 0
h-index: 0
机构:
Wenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Peoples R ChinaWenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Yu, Kehe
[1
,2
]
Cheng, Shaowen
论文数: 0引用数: 0
h-index: 0
机构:
Hainan Med Coll, Affiliated Hosp, Ctr Trauma, Haikou 570100, Peoples R ChinaWenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Cheng, Shaowen
[4
]
Peng, Lei
论文数: 0引用数: 0
h-index: 0
机构:
Hainan Med Coll, Affiliated Hosp, Ctr Trauma, Haikou 570100, Peoples R ChinaWenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
Peng, Lei
[4
]
机构:
[1] Wenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China
[2] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Peoples R China
[3] Wenzhou Med Univ, Affiliated Hosp 1, Dept Ultrasound, Wenzhou 325000, Peoples R China
[4] Hainan Med Coll, Affiliated Hosp, Ctr Trauma, Haikou 570100, Peoples R China
Myricetina flavonoid compound, has been reported to possess antioxidative, antiproliferative and antiinflammatory effects. However, no study has yet investigated the effect of myricetin on osteogenic differentiation of human bone marrow stem cells (hBMSCs). This study was designed to investigate the effects of myricetin on osteogenic differentiation of hBMSCs in vitro. Cell viability was analyzed by MTT and osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity assay. Alizarin red S dye, real time-polymerase chain reaction (RT-PCR) and Western blot analysis. We found that the ALP acrivity and the mineralization of hBMSCs were enhanced by treatement with myricetin. Myricetin increased the mRNA expressions of Osteocalcin (OCN), Collagen type I (COL I). ALP and Runt related transcription factor 2 (RUNX2). Additionally, we found that L myricetin activated the Wnt/beta-catenin pathway and increased he expression of several downsstream genes including T-cell factor-1(TCF-1) and lymphoid enhancer factor-1 (LEF-1). Depletion of beta-catenin almost completely blocked the positive role of myricetin on osteogenic differentiation. Taken together, our findings suggest that myricetin enhanced osteogenic differentiation of hBMSCs by activating the Wnt/beta-catenin signaling. The study may aid in the development of a therapeutic approach utilizing myricetin for the enhancement of bone health and prevention of osteoporosis. (C) 2014 Elsevier B.V. All rights reserved.
机构:
Kyung Hee Univ, Sch Dent, Dept Maxillofacial Biomed Engn, Seoul 130701, South Korea
Kyung Hee Univ, Sch Dent, Inst Oral Biol, Seoul 130701, South KoreaKyung Hee Univ, Sch Dent, Dept Maxillofacial Biomed Engn, Seoul 130701, South Korea
Qiao, Ling Juan
Kang, Kyung Lhi
论文数: 0引用数: 0
h-index: 0
机构:
Kyung Hee Univ, Sch Dent, Dept Periodontol, Seoul 130701, South KoreaKyung Hee Univ, Sch Dent, Dept Maxillofacial Biomed Engn, Seoul 130701, South Korea
Kang, Kyung Lhi
Heo, Jung Sun
论文数: 0引用数: 0
h-index: 0
机构:
Kyung Hee Univ, Sch Dent, Dept Maxillofacial Biomed Engn, Seoul 130701, South Korea
Kyung Hee Univ, Sch Dent, Inst Oral Biol, Seoul 130701, South KoreaKyung Hee Univ, Sch Dent, Dept Maxillofacial Biomed Engn, Seoul 130701, South Korea
机构:
China Med Univ, Dept Orthopaed, Shengjing Hosp, Shenyang, Liaoning, Peoples R ChinaChina Med Univ, Dept Orthopaed, Shengjing Hosp, Shenyang, Liaoning, Peoples R China
Wu, Dong
Piao, Longhuan
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Sch Life Sci, Shanghai, Peoples R ChinaChina Med Univ, Dept Orthopaed, Shengjing Hosp, Shenyang, Liaoning, Peoples R China
Piao, Longhuan
Wang, Guangbin
论文数: 0引用数: 0
h-index: 0
机构:
China Med Univ, Dept Orthopaed, Shengjing Hosp, Shenyang, Liaoning, Peoples R ChinaChina Med Univ, Dept Orthopaed, Shengjing Hosp, Shenyang, Liaoning, Peoples R China