Vibrational spectroscopy in stem cell characterisation: is there a niche?

被引:21
作者
Sule-Suso, J. [1 ,2 ]
Forsyth, N. R. [2 ]
Untereiner, V. [3 ,4 ]
Sockalingum, G. D. [3 ,4 ]
机构
[1] Univ Hosp North Staffordshire, Ctr Canc, Stoke On Trent ST4 6QG, Staffs, England
[2] Keele Univ, Guy Hilton Res Ctr, Inst Sci & Technol Med, Stoke On Trent ST4 7QB, Staffs, England
[3] Univ Reims, MeDIAN Biophoton & Technol Sante, UFR Pharm, F-51096 Reims, France
[4] CNRS, UMR7369, Matrice Extracellulaire & Dynann Cellulaire MEDyC, Reims, France
基金
英国工程与自然科学研究理事会;
关键词
stem cells; Raman spectroscopy; infrared spectroscopy; embryonic stem cell; mesenchymal stem cell; INDIVIDUAL LIVING CELLS; INFRARED MICROSPECTROSCOPY; RAMAN MICROSPECTROSCOPY; MIE SCATTERING; LINEAGE COMMITMENT; ENGINEERED BONE; IN-VITRO; DIFFERENTIATION; THERAPY; MICROSCOPY;
D O I
10.1016/j.tibtech.2014.03.002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Vibrational spectroscopy using both infrared and Raman spectroscopies has been used in recent years with the aim to aid clinicians in disease diagnosis. More recently, these techniques have been applied to study stem cell differentiation and to determine stem cell presence in tissues. These studies have demonstrated the potential of these techniques in better characterising stem cell differentiation phenotypes with potential applications in tissue engineering strategies. However, before the translation of vibrational spectroscopy into clinical practice becomes a reality, several issues still need to be addressed. We describe here an overview of the work carried out so far and the problems that might be encountered when using vibrational spectroscopy.
引用
收藏
页码:254 / 262
页数:9
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