Proteome analysis of human hair shaft - From protein identification to posttranslational modification

被引：103
作者：
Lee, YJ
Rice, RH [1 ]
Lee, YM
机构：
[1] Univ Calif Davis, Mol Struct Facil, Davis, CA 95616 USA
[2] Univ Calif Davis, Dept Environm Toxicol, Davis, CA 95616 USA
来源：
MOLECULAR & CELLULAR PROTEOMICS | 2006年 / 5卷 / 05期
关键词：
D O I：
10.1074/mcp.M500278-MCP200
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The human hair proteome was investigated using two-dimensional LC-MS/MS. Among the 343 identified proteins, 70 were detected in high relative abundance, including keratin intermediate filament proteins, largely extractable with denaturants. Over 300 proteins were found to constitute the insoluble complex formed by transglutaminase cross-linking. The intracellular distribution of identified proteins is wide from cytoplasm to nucleus, mitochondria, ribosome, and plasma membrane. These results help rationalize ultrastructural features visible in the mature hair. Keratins and several substrates for transglutaminase were found to be posttranslationally modified by methylation and dimethylation. Evidence for ubiquitination of hair proteins was also obtained.
引用
收藏
页码：789 / 800
页数：12
相关论文
共 50 条
  • [31] Mass spectrometric identification of posttranslational modifications in transthyretin from human blood
    I. A. Popov
    N. L. Starodubtseva
    M. I. Indeikina
    Yu. I. Kostyukevich
    A. S. Kononikhin
    M. I. Nikolaeva
    E. N. Kukaev
    S. A. Kozin
    A. A. Makarov
    E. N. Nikolaev
    Molecular Biology, 2013, 47 : 885 - 893
  • [32] The human vitreous proteome: Spatial analysis of protein expression
    Neal, RE
    Bettelheim, FA
    Garland, D
    Campbell-Winn, K
    Zigler, JS
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2004, 45 : U324 - U324
  • [33] Human hair identification by instrumental neutron activation analysis
    Cho, SY
    Jang, SG
    Chung, YS
    JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, 1998, 229 (1-2) : 143 - 147
  • [34] beta-methylthio-aspartic acid: Identification of a novel posttranslational modification in ribosomal protein S12 from Escherichia coli
    Kowalak, JA
    Walsh, KA
    PROTEIN SCIENCE, 1996, 5 (08) : 1625 - 1632
  • [35] Human hair identification by instrumental neutron activation analysis
    S. Y. Cho
    S. G. Jang
    Y. S. Chung
    Journal of Radioanalytical and Nuclear Chemistry, 1998, 229 : 143 - 147
  • [36] Identification of proteome, antigen protein and antigen membrane protein from Spiroplasma eriocheiris
    Liu, Y.
    Xu, Y.
    Li, S.
    Xu, X.
    Gao, Q.
    Yuan, M.
    Gu, W.
    Wang, W.
    Meng, Q.
    LETTERS IN APPLIED MICROBIOLOGY, 2017, 65 (05) : 395 - 402
  • [37] In-Situ imaging mass spectrometry analysis of melanin granules in the human hair shaft
    Hallégot, P
    Peteranderl, R
    Lechene, C
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2004, 122 (02) : 381 - 386
  • [38] Human hair shaft proteomic profiling: individual differences, site specificity and cuticle analysis
    Laatsch, Chelsea N.
    Durbin-Johnson, Blythe P.
    Rocke, David M.
    Mukwana, Sophie
    Newland, Abby B.
    Flagler, Michael J.
    Davis, Michael G.
    Eigenheer, Richard A.
    Phinney, Brett S.
    Rice, Robert H.
    PEERJ, 2014, 2
  • [39] Bioinformatic Analysis of the Perilymph Proteome to Generate a Human Protein Atlas
    van Dieken, Alina
    Staecker, Hinrich
    Schmitt, Heike
    Harre, Jennifer
    Pich, Andreas
    Rossberg, Willi
    Lenarz, Thomas
    Durisin, Martin
    Warnecke, Athanasia
    FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2022, 10
  • [40] Co-regulation map of the human proteome enables identification of protein functions
    Georg Kustatscher
    Piotr Grabowski
    Tina A. Schrader
    Josiah B. Passmore
    Michael Schrader
    Juri Rappsilber
    Nature Biotechnology, 2019, 37 : 1361 - 1371
12345