Green conversion of graphene oxide to graphene nanosheets and its biosafety study

被引：38
作者：
Dasgupta, Adhiraj [1 ]
Sarkar, Joy [1 ,2 ]
Ghosh, Manosij [3 ]
Bhattacharya, Amartya [4 ]
Mukherjee, Anita [3 ]
Chattopadhyay, Dipankar [4 ]
Acharya, Krishnendu [1 ,5 ]
机构：
[1] Univ Calcutta, Dept Bot, Mol & Appl Mycol & Plant Pathol Lab, Kolkata, W Bengal, India
[2] Dinabandhu Andrews Coll, Dept Bot, Kolkata, W Bengal, India
[3] Univ Calcutta, Cell Biol & Genet Toxicol Lab, Ctr Adv Study Cell & Chromosome Res, Dept Bot, Kolkata, W Bengal, India
[4] Univ Calcutta, Dept Polymer Sci & Technol, Univ Coll Sci & Technol, Kolkata, W Bengal, India
[5] Univ Calcutta, Ctr Res Nanosci & Nanotechnol, Technol Campus, Kolkata, W Bengal, India
来源：
PLOS ONE | 2017年 / 12卷 / 02期
关键词：
WALLED CARBON NANOTUBES; ECTOMYCORRHIZAL EDIBLE MUSHROOM; DIOXIDE TIO2 NANOPARTICLES; EXFOLIATED GRAPHITE OXIDE; MS/MS PROTEOME ANALYSIS; HEPATOMA HEPG2 CELLS; ALBONIGRA KROMBH. FR; IN-VITRO; SILVER NANOPARTICLES; DNA-DAMAGE;
D O I：
10.1371/journal.pone.0171607
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Chemical reduction of graphene oxide (GO) to graphene employs the use of toxic and environmentally harmful reducing agents, hindering mass production of graphene which is of tremendous technological importance. In this study we report a green approach to the synthesis of graphene, bio-reduced by crude polysaccharide. The polysaccharide reduces exfoliated GO to graphene at room temperature in an aqueous medium. Transmission electron microscopy image provides clear evidence for the formation of few layer graphene. Characterization of the resulting polysaccharide reduced GO by Raman spectroscopy, Fourier transform infrared spectroscopy and Energy dispersive X-ray analysis confirms reduction of GO to graphene. We also investigated the degree of biosafety of the reduced GO and found it to be safe under 100 mu g/ml.
引用
收藏
页数：20
相关论文
共 78 条
[1]   A NEW RAPID AND SIMPLE NONRADIOACTIVE ASSAY TO MONITOR AND DETERMINE THE PROLIFERATION OF LYMPHOCYTES - AN ALTERNATIVE TO [H-3] THYMIDINE INCORPORATION ASSAY [J].
AHMED, SA ;
GOGAL, RM ;
WALSH, JE .
JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 170 (02) :211-224
[2]   Genotoxicity of graphene nanoribbons in human mesenchymal stem cells [J].
Akhavan, Omid ;
Ghaderi, Elham ;
Emamy, Hamed ;
Akhavan, Fatima .
CARBON, 2013, 54 :419-431
[3]   Size-dependent genotoxicity of graphene nanoplatelets in human stem cells [J].
Akhavan, Omid ;
Ghaderi, Elham ;
Akhavan, Alireza .
BIOMATERIALS, 2012, 33 (32) :8017-8025
[4]   Ultrathin epitaxial graphite: 2D electron gas properties and a route toward graphene-based nanoelectronics [J].
Berger, C ;
Song, ZM ;
Li, TB ;
Li, XB ;
Ogbazghi, AY ;
Feng, R ;
Dai, ZT ;
Marchenkov, AN ;
Conrad, EH ;
First, PN ;
de Heer, WA .
JOURNAL OF PHYSICAL CHEMISTRY B, 2004, 108 (52) :19912-19916
[5]   TOXICITY DETERMINED INVITRO BY MORPHOLOGICAL ALTERATIONS AND NEUTRAL RED ABSORPTION [J].
BORENFREUND, E ;
PUERNER, JA .
TOXICOLOGY LETTERS, 1985, 24 (2-3) :119-124
[6]   ISOLATION OF LYMPHOCYTES, GRANULOCYTES AND MACROPHAGES [J].
BOYUM, A .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1976, :9-15
[7]   A distinct utility of the amide III infrared band for secondary structure estimation of aqueous protein solutions using partial least squares methods [J].
Cai, SW ;
Singh, BR .
BIOCHEMISTRY, 2004, 43 (09) :2541-2549
[8]   A Facile One-step Method to Produce Graphene-CdS Quantum Dot Nanocomposites as Promising Optoelectronic Materials [J].
Cao, Aoneng ;
Liu, Zhen ;
Chu, Saisai ;
Wu, Minghong ;
Ye, Zhangmei ;
Cai, Zhengwei ;
Chang, Yanli ;
Wang, Shufeng ;
Gong, Qihuang ;
Liu, Yuanfang .
ADVANCED MATERIALS, 2010, 22 (01) :103-+
[9]   An environment-friendly preparation of reduced graphene oxide nanosheets via amino acid [J].
Chen, Dezhi ;
Li, Lidong ;
Guo, Lin .
NANOTECHNOLOGY, 2011, 22 (32)
[10]   Gold Nanoparticles: Microbial Synthesis and Application in Water Hygiene Management [J].
Das, Sujoy K. ;
Das, Akhil R. ;
Guha, Arun K. .
LANGMUIR, 2009, 25 (14) :8192-8199
12345678