Directed Evolution of Multivalent Glycopeptides Tightly Recognized by HIV Antibody 2G12

被引：70

作者：

Horiya, Satoru ^{[1
]}

Bailey, Jennifer K. ^{[1
]}

Temme, J. Sebastian ^{[1
]}

Schippe, Yollete V. Guillen ^{[2
,3
]}

Krauss, Isaac J. ^{[1
]}

机构：

[1] Brandeis Univ, Dept Chem, Waltham, MA 02454 USA

[2] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA

[3] Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Boston, MA 02114 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2014年 / 136卷 / 14期

关键词：

IMMUNODEFICIENCY-VIRUS TYPE-1; IN-VITRO SELECTION; MESSENGER-RNA DISPLAY; VACCINE DESIGN; DC-SIGN; OLIGOMANNOSE CLUSTERS; CARBOHYDRATE EPITOPE; PURIFIED COMPONENTS; RIBOSOMAL SYNTHESIS; UNNATURAL PEPTIDES;

D O I：

10.1021/ja500678v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein, we report a method for in vitro selection of multivalent glycopeptides, combining mRNA display with incorporation of unnatural amino acids and "click" chemistry. We have demonstrated the use of this method to design potential glycopeptide vaccines against HIV. From libraries of similar to 10(13) glycopeptides containing multiple Man(9) glycan(s), we selected variants that bind to HIV broadly neutralizing antibody 2G12 with picomolar to low nanomolar affinity. This is comparable to the strength of the natural 2G12-gp120 interaction, and is the strongest affinity achieved to date with constructs containing 3-5 glycans. These glycopeptides are therefore of great interest in HIV vaccine design.

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收藏

页码：5407 / 5415

页数：9

相关论文

共 61 条

[1] Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor, the Octet [J].

Abdiche, Yasmina ;

Malashock, Dan ;

Pinkerton, Alanna ;

Pons, Jaurne .

ANALYTICAL BIOCHEMISTRY, 2008, 377 (02) :209-217

[2] Yeast-Elicited Cross-Reactive Antibodies to HIV Env Glycans Efficiently Neutralize Virions Expressing Exclusively High-Mannose N-Linked Glycans [J].

Agrawal-Gamse, Caroline ;

Luallen, Robert J. ;

Liu, Bingfen ;

Fu, Hu ;

Lee, Fang-Hua ;

Geng, Yu ;

Doms, Robert W. .

JOURNAL OF VIROLOGY, 2011, 85 (01) :470-480

[3] A monosaccharide-modified peptide phage library for screening of ligands to carbohydrate-binding proteins [J].

Arai, Kanako ;

Tsutsumi, Hiroshi ;

Mihara, Hisakazu .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2013, 23 (17) :4940-4943

[4] Site-selective modification of proteins for the synthesis of structurally defined multivalent scaffolds [J].

Artner, Lukas M. ;

Merkel, Lars ;

Bohlke, Nina ;

Beceren-Braun, Figen ;

Weise, Christoph ;

Dernedde, Jens ;

Budisa, Nediljko ;

Hackenberger, Christian P. R. .

CHEMICAL COMMUNICATIONS, 2012, 48 (04) :522-524

[5] A glycoconjugate antigen based on the recognition motif of a broadly neutralizing human immunodeficiency virus antibody, 2G12, is immunogenic but elicits antibodies unable to bind to the self glycans of gp120 [J].

Astronomo, Rena D. ;

Lee, Hing-Ken ;

Scanlan, Christopher N. ;

Pantophlet, Ralph ;

Huang, Cheng-Yuan ;

Wilson, Ian A. ;

Blixt, Ola ;

Dwek, Raymond A. ;

Wong, Chi-Huey ;

Burton, Dennis R. .

JOURNAL OF VIROLOGY, 2008, 82 (13) :6359-6368

[6] Defining Criteria for Oligomannose Immunogens for HIV Using Icosahedral Virus Capsid Scaffolds [J].

Astronomo, Rena D. ;

Kaltgrad, Eiton ;

Udit, Andrew K. ;

Wang, Sheng-Kai ;

Doores, Katie J. ;

Huang, Cheng-Yuan ;

Pantophlet, Ralph ;

Paulson, James C. ;

Wong, Chi-Huey ;

Finn, M. G. ;

Burton, Dennis R. .

CHEMISTRY & BIOLOGY, 2010, 17 (04) :357-370

[7] Comprehensive cross-clade neutralization analysis of a panel of anti-human immunodeficiency virus type 1 monoclonal antibodies [J].

Binley, JA ;

Wrin, T ;

Korber, B ;

Zwick, MB ;

Wang, M ;

Chappey, C ;

Stiegler, G ;

Kunert, R ;

Zolla-Pazner, S ;

Katinger, H ;

Petropoulos, CJ ;

Burton, DR .

JOURNAL OF VIROLOGY, 2004, 78 (23) :13232-13252

[8] Antibody domain exchange is an immunological solution to carbohydrate cluster recognition [J].

Calarese, DA ;

Scanlan, CN ;

Zwick, MB ;

Deechongkit, S ;

Mimura, Y ;

Kunert, R ;

Zhu, P ;

Wormald, MR ;

Stanfield, RL ;

Roux, KH ;

Kelly, JW ;

Rudd, PM ;

Dwek, RA ;

Katinger, H ;

Burton, DR ;

Wilson, IA .

SCIENCE, 2003, 300 (5628) :2065-2071

[9] Dissection of the carbohydrate specificity of the broadly neutralizing-anti-HIV-1 antibody 2G12 [J].

Calarese, DA ;

Lee, HK ;

Huang, CY ;

Best, MD ;

Astronomo, RD ;

Stanfield, RL ;

Katinger, H ;

Burton, DR ;

Wong, CH ;

Wilson, IA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (38) :13372-13377

[10]

Chan W., 1999, Fmoc solid phase peptide synthesis: a practical approach

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