Genotyping and strain distribution of Mycobacterium avium subspecies hominissuis isolated from humans and pigs in Belgium, 2011 2013

被引:0
作者
Vluggen, C. [1 ]
Soetaert, K. [1 ]
Duytschaever, L. [2 ,3 ]
Denoel, J. [2 ]
Fauville-Dufaux, M. [1 ]
Smeets, F. [2 ]
Bruffaerts, N. [4 ]
Huygen, K. [4 ]
Fretin, D. [3 ]
Rigouts, L. [5 ,6 ]
Saegerman, C. [2 ]
Mathys, V. [1 ]
机构
[1] Sci Inst Publ Hlth WIV ISP, Bacterial Dis Serv, Operat Direct Communicable & Infect Dis, Brussels, Belgium
[2] FARAH, Res Unit Epidemiol & Risk Anal Appl Vet Sci UREAR, Liege, Belgium
[3] Vet & Agrochem Res Ctr CODA CERVA, Bacterial Zoonoses Livestock Unit, Operat Direct Bacterial Dis, Brussels, Belgium
[4] Sci Inst Publ Hlth WIV ISP, Serv Immunol, Operat Direct Communicable & Infect Dis, Brussels, Belgium
[5] Inst Trop Med, Mycobacteriol Unit, B-2000 Antwerp, Belgium
[6] Univ Antwerp, Dept Biomed Sci, Antwerp, Belgium
来源
EUROSURVEILLANCE | 2016年 / 21卷 / 03期
关键词
NONTUBERCULOUS MYCOBACTERIA; IDENTIFICATION; PARATUBERCULOSIS; SEQUENCE; INFECTION; OUTBREAK; DISEASE;
D O I
暂无
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Mycobacterium avium represents a health concern for both humans and pigs. The characterisation of its subspecies is an important step improving the understanding of the epidemiology and the control of this pathogen. Ninety-two human M. avium strains were selected fora retrospective study. Subspecies determination by rpoD sequencing and 151245/15901 analysis showed that 98.9% of Belgian human M. 01/10177 strains belong to the subspecies hominissuis (MAH). Some of these MAH strains present particular 151245/15901 profiles (absence of 151245 and false 15901 detection provoked by the presence of ISMov6). In addition, 54 MAH strains isolated from submandibular lymph nodes of Belgian pigs with lymphadenitis were included in this study. Genotyping of human and porcine isolates was performed using multispacer sequence typing (MST). In total, 49 different MST types were identified among pig (n= n) and human (n similar to 4]) MA isolates, with only five shared by both hosts. Among these MST types, 34 were newly identified. Our findings demonstrate the extensive genetic diversity among MAH isolates. Some genotypes were more prevalent in human or pigs but no correlation was observed between MST type and place of residence or the farm of origin for human and porcine isolates respectively, suggesting an environmental source of infection.
引用
收藏
页码:18 / 25
页数:8
相关论文
共 33 条
  • [1] Investigation of an outbreak of mycobacteriosis in pigs
    Agdestein, Angelika
    Johansen, Tone B.
    Polacek, Vladimir
    Lium, Bjorn
    Holstad, Gudmund
    Vidanovic, Dejan
    Aleksic-Kovacevic, Sanja
    Jorgensen, Anne
    Zultauskas, Jonas
    Nilsen, Sigrun F.
    Djonne, Berit
    [J]. BMC VETERINARY RESEARCH, 2011, 7
  • [2] Epidemiological investigation of a Mycobacterium avium subsp. hominissuis outbreak in swine
    Alvarez, J.
    Castellanos, E.
    Romero, B.
    Aranaz, A.
    Bezos, J.
    Rodriguez, S.
    Mateos, A.
    Dominguez, L.
    De Juan, L.
    [J]. EPIDEMIOLOGY AND INFECTION, 2011, 139 (01) : 143 - 148
  • [3] Species identification of Mycobacterium avium complex isolates by a variety of molecular techniques
    Beggs, ML
    Stevanova, R
    Eisenach, KD
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (02) : 508 - 512
  • [4] rpoB sequence-based identification of Mycobacterium avium complex species
    Ben Salah, Iskandar
    Adekambi, Toidi
    Raoult, Didier
    Drancourt, Michel
    [J]. MICROBIOLOGY-SGM, 2008, 154 : 3715 - 3723
  • [5] Genotyping of Mycobacterium avium complex organisms using multispacer sequence typing
    Cayrou, Caroline
    Turenne, Christine
    Behr, Marcel A.
    Drancourt, Michel
    [J]. MICROBIOLOGY-SGM, 2010, 156 : 687 - 694
  • [6] Nontuberculous mycobacteria: opportunistic environmental pathogens for predisposed hosts
    Cook, James L.
    [J]. BRITISH MEDICAL BULLETIN, 2010, 96 (01) : 45 - 59
  • [7] Mycobacterial infection of pigs in Croatia
    Cvetnic, Z.
    Spicic, S.
    Benic, M.
    Katalinic-Jankovic, Vera
    Pate, Mateja
    Krt, B.
    Ocepek, M.
    [J]. ACTA VETERINARIA HUNGARICA, 2007, 55 (01) : 1 - 9
  • [8] Diversity of Mycobacterium avium subsp hominissuis mycobacteria causing lymphadenitis, France
    Despierres, L.
    Cohen-Bacrie, S.
    Richet, H.
    Drancourt, M.
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2012, 31 (07) : 1373 - 1379
  • [9] Shared Mycobacterium avium Genotypes Observed among Unlinked Clinical and Environmental Isolates
    Dirac, M. Ashworth
    Weigel, Kris M.
    Yakrus, Mitchell A.
    Becker, Annie L.
    Chen, Hui-Ling
    Fridley, Gina
    Sikora, Arthur
    Speake, Cate
    Hilborn, Elizabeth D.
    Pfaller, Stacy
    Cangelosi, Gerard A.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2013, 79 (18) : 5601 - 5607