Pyrosequencing analysis of SNCA methylation levels in leukocytes from Parkinson's disease patients

被引：39
作者：
Song, Yang [1 ,2 ]
Ding, Hui [1 ,2 ]
Yang, Jingfang [2 ]
Lin, Qingling [1 ,2 ]
Xue, Jinhua [1 ,2 ]
Zhang, Yanli [1 ,2 ]
Chan, Piu [1 ,2 ]
Cai, Yanning [1 ,2 ]
机构：
[1] Capital Med Univ, Xuanwu Hosp, Dept Neurol, Key Lab Neurodegenerat Dis,Minist Educ, Beijing 100053, Peoples R China
[2] Capital Med Univ, Xuanwu Hosp, Dept Neurobiol, Key Lab Neurodegenerat Dis,Minist Educ, Beijing 100053, Peoples R China
来源：
NEUROSCIENCE LETTERS | 2014年 / 569卷
基金：
中国国家自然科学基金;
关键词：
alpha-Synuclein; DNA methylation; Pyrosequencing; Leukocytes; ALPHA-SYNUCLEIN; NEURONS; DNA; TRIPLICATION; DUPLICATION; PROMOTER;
D O I：
10.1016/j.neulet.2014.03.076
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
DNA methylation of a CpG island located in intron 1 of the alpha-synuclein gene (SNCA) has been reported to play an essential role in the regulation of alpha-synuclein transcription, and probably has a close association with Parkinson's disease (PD). However, there is no simple and cost effective method to quantify DNA methylation in this region. Additionally, whether this CpG island is hypomethylated in peripheral blood in PD and can be used as PD biomarker is still under debate. In the present study, we developed a set of bisulfite pyrosequencing assays which can be used to examine the DNA methylation level of 13 CpG sites in intron 1 of SNCA. We compared DNA methylation levels at these sites in leukocytes from 50 PD patients and 50 healthy controls. Our results indicated that there were no significant differences in DNA methylation between PD patients and controls. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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收藏
页码：85 / 88
页数：4
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